Description

5-(3-aminoallyl)-2′-deoxyuridine 5′-triphosphate

AA-dUTPcan be used for enzymatic indirect non-radioactive labeling of DNA in PCR, nick-translation, cDNA synthesis or primer extension reactions. It can be incorporated into DNA using Reverse Transcriptases, Taq DNA Polymerase, Klenow Fragment. The resulting aminoallyl-containing DNA can be subsequently labeled with any amine-reactive fluorescent dye, biotin or hapten, resulting in a uniform and high density labeling of DNA.

Storage Condition

-20°C .

Concentration

1 µmol (100 µl of 10 mM solution)

Purity

96-99% (HPLC).