APPLICATION

1. Visualization of DNA bands as they separate during agarose gel electrophoresis;

2. Isolation of DNA fragments for subclonning without introducing mutations normally caused by EB and ultraviolet.

Description

Glow-Green is an environment safe non-EB nucleic acid stain used for detecting double-stranded DNA, single-stranded DNA, and RNA in agarose gels. This new stain has two fluorescence excitation maxima when bound to nucleic acid, one centered at approximately 290nm and one at approximately 490nm.

Glow-Green is as sensitive as EB, and visualization of DNA band (>50 ng) is possible under blue (best) or visible light.

Compared to EB known as a strong mutagen, Glow-Green causes much fewer mutations in the Ames test. In addition, Glow-Green has a negative test in mouse marrow chromophilous erythrocyte micronucleus test and mouse spermatocyte chromosomal aberration test. Glow-Green is a environment-friendly non-EB stain for detecting nucleic acid in agarose gels, especially when isolating DNA fragment for subclonning.

CONTENTS

1 ml (20,000x), sufficient to prepare 20 liter agarose gel running buffer.