Cat#: CA-C315

Name

Elite^TM cAMP ELISA Assay Kit (Fluorescence)

Description

Elite™ cAMP ELISA Assay Kit provides the sensitive method for detecting adenylate cyclase activity in biochemical or cell-based assay system. Compared with other ELISA cAMP assay kits, our kit eliminates the tedious acetylation step. The kit uses Elite™ Red as a fluorimetric substrate to quantify the HRP activity. The high sensitivity (as low as 0.1 nM) make it superior to any other known kits that are colorimetric-based or fluorescence-based.

Application

The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. The fluorescent product formed is proportional to the activity of HRP-cAMP conjugate.

Sensitivity

0.1 pmol/ml to 10 pmol/ml; EC₅₀ = 1.6 pmol/ml

Size

1 plate (96-well)

Detection

Fluorescence microplate reader