CA-K045

Name

Elite^TM Kinase Activity Assay Kit (Red Fluorescence).

Description

The Elite™ Kinase Activity Assay Kit is based on monitoring ADP formation, which is directly proportional to enzyme phosphotransferase activity and is measured fluorimetrically. This enzyme-coupled kit provides a fast, simple, and homogeneous assay to measure kinase activities. It is a non-radioactive and no wash method to detect the amount of ADP produced from enzyme reaction.

Application

The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step.

The high sensitivity (<0.3 μM ADP) and broad ATP tolerance (1-300 μM) make it an ideal kit for determining kinase Michaelis-Menten kinetics and for screening and identifying kinase inhibitors.

Size

250 assays in 96-well plates.

Ex/Em

540/590 nm.

Detection

Fluorescence microplate reader.