CA-N405

Name

Elite^TM NADPH Assay Kit (Red Fluorescence)

Description

The Elite™ Fluorimetric NADPH Assay Kit provides a sensitive, one-step assay to detect as little as 100 picomoles of NADPH in a 100 μl assay volume (1 μM). It has very low background since it is run in the red visible range that significantly reduces the interference resulted from biological samples.

Application

The assay can be performed in a convenient 96-well or 384-well microtiter-plate format. Its signal can be easily read by either a fluorescence microplate reader at Ex/Em = 540/590 nm or an absorbance microplate reader at ~576 nm.

This kit has been used for screening enzyme activities that use NADP/NADPH as a cofactor. It has also been used for the sensitive detection of NADPH in cell-based assays. Compared to the other commercial kits, this assay has higher signal/background ratio.

Size

400 assays in 96-well plates

Ex/Em

540/590 nm

Detection

Fluorescence microplate reader