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Guide for selecting Taq DNA Polymerases for Your Nucleic Acid Amplification

 

Polymerase Chain Reaction (PCR), invented by Kary B. Mullis, at the Cetus Corporation, who was awarded the 1993 Nobel Prize for chemistry for PCR, is a technique to exponentially amplify in vitro a small quantity of a specific nucleotide sequence using a thermostable (Taq) DNA polymerase. The DNA polymerase reads the template and incorporates a complimentary nucleotide yielding a newly assembled complimentary strand.

 

eENZYME provides high quality and innovative Taq DNA Polymerases for the amplification of any DNA sequences from mammalians, plants, bacteria, or virus. eENZYME Taq DNA Polymerases have been specifically modified or proportionally mixed to fit for various DNA amplifications at variety of conditions.

 

The following tables are used as references for you to select a specific Taq DNA Polymerase/PCR Ready Mix for your various PCR amplifications.

 

Catalogue#

Product

Fidelity

Specificity

GC-rich Amplifying

Hot Start

Amplifying Size (genomic DNA)

DP-001-0050

SupraTaq

+++

+++

No

No

<3 kb

DP-002-0050

BioTherm Taq

++++

++++

No

No

<10 kb

DP-016-0250

PCR Ready Master Mix

+++++

+++++

Yes

Yes

<5kb

DP-005-0050

EU-Taq

+++++

++++

Yes

No

<5 kb

DP-003-0050

High Fidelity Taq

+++++++++

++++

No

No

<2 kb

Note: for high fidelity DNA amplification, we suggest to use our High Fidelity Taq that has the same accuracy rate of PfuDNA Polymerase.

PCR Protocol