Guide for selecting Taq
DNA Polymerases for
Your Nucleic Acid Amplification
Polymerase
Chain Reaction (PCR), invented by Kary B. Mullis, at the Cetus Corporation, who
was awarded the 1993 Nobel Prize for chemistry for PCR, is a technique to
exponentially amplify in vitro a small quantity of a specific nucleotide
sequence using a thermostable (Taq) DNA polymerase. The DNA polymerase reads the
template and incorporates a complimentary nucleotide yielding a newly assembled
complimentary strand.
eENZYME provides high quality
and innovative Taq DNA Polymerases for the amplification of any DNA sequences
from mammalians, plants, bacteria, or virus. eENZYME Taq DNA Polymerases have
been specifically modified or proportionally mixed to fit for various DNA
amplifications at variety of conditions.
The following tables are used as
references for you to select a specific Taq DNA Polymerase/PCR Ready Mix for
your various PCR amplifications.
|
Catalogue#
|
Product
|
Fidelity
|
Specificity
|
GC-rich Amplifying
|
Hot Start
|
Amplifying Size (genomic DNA)
|
|
DP-001-0050
|
SupraTaq
|
+++
|
+++
|
No
|
No
|
<3 kb
|
|
DP-002-0050
|
BioTherm Taq
|
++++
|
++++
|
No
|
No
|
<10 kb
|
|
DP-016-0250
|
PCR Ready Master Mix
|
+++++
|
+++++
|
Yes
|
Yes
|
<5kb
|
|
DP-005-0050
|
EU-Taq
|
+++++
|
++++
|
Yes
|
No
|
<5 kb
|
|
DP-003-0050
|
High Fidelity Taq
|
+++++++++
|
++++
|
No
|
No
|
<2 kb
|
Note:
for high fidelity DNA
amplification, we suggest to use our High Fidelity Taq that has the same
accuracy rate of PfuDNA Polymerase.