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Human Apoptosis qPCR Array
2 or 12 plates plus qPCR MasterMix
Price: From $199.00 to $1,199.00

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Detailed Description

This Apoptosis Array is designed to profile the expression of 91 key genes involved in the human apoptosis and 5 house-keeping genes.

Features

  • More genes featured for each pathway
  • Fits to ABI, BioRad and many other Real-Time thermocyclers
  • Strong detection signals without leaking to the adjacent wells
  • Higher sensitivity and sharper melting curve
  • All sets of primers span at least one intron of the genomic sequence with annealing temperatures of 60 ± 1 oC
  • EvaGreen PCR Mastermix (2x) included

 

Catalog Number

qPCR Array Format

Real-Time Thermal Cyclers

GA-A102A

Human Apoptosis qPCR Array Format A

Bio-Rad CFX96, CFX384; Bio-Rad/MJ Research models Chrom4, DNA Engine Bio-Rad models iCycler, iQ5, MyiQ, MyiQ2, Opticon 2; Roche LightCycler 480 (96).

GA-A102B

Human Apoptosis qPCR Array Format B

Applied Biosystems models 5700, 7300, 7500 (Standard and Fast), 7700, 7900HT (Standard and Fast); StepOnePlus, ViiA7 (Standard and Fast); Eppendorf Mastercycler ep realplex models 2, 2S, 4, 4S; Stratagene models Mx3000P, Mx3005P, Mx4000; Takara TP-800.

Gene Information

Position

GeneBank

Symbol

Name
A1  NM_003805 Cradd CASP2 and RIPK1 domain containing adaptor with death domain
A2 NM_003824 Fadd Fas (TNFRSF6)-associated via death domain
A3 NM_000594 TNF Tumor necrosis factor (TNF superfamily, member 2)
A4 NM_002342 Ltbr Lymphotoxin beta receptor (TNFR superfamily, member 3)
A5 NM_001279 Cidea Cell death-inducing DFFA-like effector a
A6 NM_014430 cideb Cell death-inducing DFFA-like effector b
A7 NM_000546 Tp53 Tumor protein p53
A8 NM_005427.3 Trp73 Tumor protein p73 (TP73)
A9 NM_003879 Cflar (Casper) CASP8 and FADD-like apoptosis regulator
A10 NM_004938 Dapk1 Death-associated protein kinase 1
A11 NM_004322 Bad BCL2-associated agonist of cell death
A12 NM_001188 Bak1 BCL2-antagonist/killer 1
B1 NM_003551.2 Nme5 Non-metastatic cells 5, protein expressed in 
B2 NM_001196 Bid BH3 interacting domain death agonist
B3 NM_004052 Bnip3 BCL2/adenovirus E1B 19kDa interacting protein 3
B4 NM_004331 Bnip3I BCL2/adenovirus E1B 19kDa interacting protein 3-like
B5 NM_004330 Bnip2 BCL2/adenovirus E1B 19kDa interacting protein 2
B6 NM_033292 Casp1 (ice) Caspase 1, apoptosis-related cysteine peptidase (interleukin 1)
B7 NM_032982 casp2 Caspase 2, apoptosis-related cysteine peptidase
B8 NM_004346 Casp 3 Caspase 3, apoptosis-related cysteine peptidase
B9 NM_001225 casp4 Caspase 4, apoptosis-related cysteine peptidase
B10 NM_004347 Casp5 Caspase 5, apoptosis-related cysteine peptidase
B11 NM_032992 casp6 Caspase 6, apoptosis-related cysteine peptidase
B12 NM_001227 Casp7 Caspase 7, apoptosis-related cysteine peptidase
C1 NM_001228 casp8 Caspase 8, apoptosis-related cysteine peptidase
C2 NM_001229 casp9 Caspase 9, apoptosis-related cysteine peptidase
C3 NM_001230 Casp10 Caspase 10, apoptosis-related cysteine peptidase
C4 NM_012114 Casp14 Caspase 14, apoptosis-related cysteine peptidase
C5 NM_001160 Apaf1 Apoptotic peptidase activating factor 1
C6 NM_004401 Dffa DNA fragmentation factor, 45kDa, alpha polypeptide
C7 NM_004402.2| Dffb DNA fragmentation factor, 40kDa, beta polypeptide 
C8 NM_004324 Bax BCL2-associated X protein
C9 NM_032515.3| Bok BCL2-related ovarian killer
C10 NM_013258 Pycard (Tms1/Asc) PYD and CARD domain containing
C11 NM_183237.2 Rnf7 Ring finger protein 7
C12 NM_003810 Tnfsf10 (Trail) Tumor necrosis factor (ligand) superfamily, member 10
D1 NM_005426 Tp53bp2 Tumor protein p53 binding protein, 2
D2 NM_033285.3 Tp53inp1 Homo sapiens tumor protein p53 inducible nuclear protein 1 
D3 NM_005157 ABL1 C-abl oncogene 1, receptor tyrosine kinase
D4 NM_005163 AKT1 V-akt murine thymoma viral oncogene homolog 1
D5 NM_006595.3 Api5 Apoptosis inhibitor 5 
D6 NM_004323 Bag1 BCL2-associated athanogene
D7 NM_004281 Bag3 BCL2-associated athanogene 3
D8 NM_004874 bag4 BCL2-associated athanogene 4
D9 NM_016561 Bfar Bifunctional apoptosis regulator
D10 NM_001197 Bik BCL2-interacting killer (apoptosis-inducing)
D11 NM_004456.4 EZH2 Enhancer of zeste homolog 2 (Drosophila) (EZH2)
D12 NM_003998.3 NFKB1 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
E1 NM_003842 TNFrsf10b (DR5) Tumor necrosis factor receptor superfamily, member 10b
E2 NM_033012.3 TNFsf12 Tumor necrosis factor (ligand) superfamily, member 11 (TNFSF11)
E3 NM_000074 CD40Ig (Tnfsf5) CD40 ligand
E4 NM_002546 TNFrsf11b Tumor necrosis factor receptor superfamily, member 11b
E5 NM_001252 cd70 (Tnfsf7) CD70 molecule
E6 NM_000043 Fas (Tnfrsf6) Fas (TNF receptor superfamily, member 6)
E7 NM_004536 Naip1 (Birc1) NLR family, apoptosis inhibitory protein
E8 NM_001167 Xiap (Birc4) X-linked inhibitor of apoptosis
E9 NM_001165 Birc3 (c-IAP1) Baculoviral IAP repeat-containing 3
E10 NM_001168.2 Birc5 Baculoviral IAP repeat containing 5 (BIRC5)
E11 NM_016252 birc6 Baculoviral IAP repeat-containing 6
E12 NM_005011.3 NRF1 Nuclear respiratory factor 1
F1 NM_003946 Nol3 Nucleolar protein 3 (apoptosis repressor with CARD domain)
F2 NM_177990.2 Pak7 P21 protein (Cdc42/Rac)-activated kinase 7 (
F3 NM_006875.3 Pim2 serine/threonine-protein kinase pim-2
F4 NM_002690.1 Polb PREDICTED: polymerase (DNA directed), beta isoform 3
F5 NM_181738.1 Prdx2 Homo sapiens peroxiredoxin 2 (PRDX2), nuclear gene encoding mitochondrial protein
F6 NM_020126.3 Sphk2 Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant 1, mRNA
F7 NM_002392.3 MDM2 Homo sapiens Mdm2 p53 binding protein homolog (mouse) (MDM2), transcript variant MDM2
F8 NM_021960 Mcl1 Myeloid cell leukemia sequence 1 (BCL2-related)
F9 NM_003403.3 YY1 Homo sapiens YY1 transcription factor (YY1),
F10 NM_013261.3 PGC Homo sapiens peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
F11 NM_004958.3 mTor Homo sapiens mechanistic target of rapamycin (serine/threonine kinase) (MTOR),
F12 NM_000474.3 TWIST1 (Twist) Homo sapiens twist homolog 1 (Drosophila) (TWIST1),
G1 NM_003921 Bcl10 B-cell CLL/lymphoma 10
G2 NM_004049 Bcl2a1 BCL2-related protein A1
G3 NM_006538 Bcl2l11 BCL2-like 11 (apoptosis facilitator)
G4 NM_014739 Bclaf1 BCL2-associated transcription factor 1
G5 NM_000633 Bcl2 B-cell CLL/lymphoma 2
G6 NM_138578 Bcl2l1 (Bcl-x) BCL2-like 1
G7 NM_020396 Bcl2l10 BCL2-like 10 (apoptosis facilitator)
G8 NM_004050 Bcl2l2 BCL2-like 2
G9 NM_001344.2 Dad1 Homo sapiens defender against cell death 1 (DAD1),
G10 NM_018063.3 Hells Homo sapiens helicase, lymphoid-specific (HELLS
G11 NM_033343.3 Lhx4 Homo sapiens LIM homeobox 4 (LHX4),
G12 NM_005985.3 SNAI1 (Snail) Homo sapiens snail homolog 1 (Drosophila) (SNAI1),
H1 NM_006092 Nod1 (Card4) Nucleotide-binding oligomerization domain containing 1
H2 NM_014550.3 Card10 Homo sapiens caspase recruitment domain family, member 10 (CARD10),
H3 NM_032587 Card6 Caspase recruitment domain family, member 6
H4 NM_014959 Card8 Caspase recruitment domain family, member 8
H5 NM_005658.4 Traf1 Homo sapiens TNF receptor-associated factor 1 (TRAF1), transcript variant 1
H6 NM_021138 Traf2 TNF receptor-associated factor 2
H7 NM_003300 Traf3 TNF receptor-associated factor 3
H8 NM_002046 GAPDH Glyceraldehyde-3-phosphate dehydrogenase
H9 NM_002046 GAPDH Glyceraldehyde-3-phosphate dehydrogenase
H10 NM_001101 ACTB Actin, beta
H11 NM_006082.2 Tubulin Homo sapiens tubulin, alpha 1b (TUBA1B),
H12 NM_000194 HPRT1 Hypoxanthine phosphoribosyltransferase 1

PCR Array Layout (96-Well)
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