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Human Fibrosis qPCR Array
12 Plates Included plus qPCR MasterMix
Price: From $199.00 to $1,199.00

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Detailed Description

This Fibrosis PCR Array was designed to profile the expression of 90 key genes involved in the human fibrosis and 6 house-keeping genes.

Features

  • More genes featured for each pathway
  • Fits to ABI, Biorad and many other Real-Time Thermocyclers
  • Strong detection signals without leaking to the adjacent wells
  • Higher sensitivity and sharper melting curve
  • All sets of primers span at least on intron of the genomic sequence with annealing temperatures of 60 ± 1 oC
  • EvaGreen PCR Mastermix (2x) included

 

Catalog Number

qPCR Array Format

Real-Time Thermal Cyclers

GA-F101A

Human Fibrosis qPCR Array Format A

Bio-Rad CFX96, CFX384; Bio-Rad/MJ Research models Chrom4, DNA Engine Bio-Rad models iCycler, iQ5, MyiQ, MyiQ2, Opticon 2; Roche LightCycler 480 (96).

GA-F101B

Human Fibrosis qPCR Array Format B

Applied Biosystems models 5700, 7300, 7500 (Standard and Fast), 7700, 7900HT (Standard and Fast); StepOnePlus, ViiA7 (Standard and Fast); Eppendorf Mastercycler ep realplex models 2, 2S, 4, 4S; Stratagene models Mx3000P, Mx3005P, Mx4000; Takara TP-800.

Gene Information

Position

GeneBank

Symbol

Name

A01 NM_001613 ACTA2 Actin
A02 NM_000029 AGT Angiotensinogen
A03 NM_005163 AKT1 V-akt murine thymoma viral oncogene homolog 1
A04 NM_000633 BCL2 B-cell CLL/lymphoma 2
A05 NM_001719 BMP7 Bone morphogenetic protein 7
A06 NM_001753 CAV1 Caveolin 1, caveolae protein, 22kDa
A07 NM_002986 CCL11 Chemokine (C-C motif) ligand 11
A08 NM_002982 CCL2 Chemokine (C-C motif) ligand 2
A09 NM_002983 CCL3 Chemokine (C-C motif) ligand 3
A10 NM_001123396 CCR2 Chemokine (C-C motif) receptor 2
A11 NM_005194 CEBPB CCAAT/enhancer binding protein (C/EBP), beta
A12 NM_000089 COL1A2 Collagen, type I, alpha 2
B01 NM_000090 COL3A1 Collagen, type III, alpha 1
B02 NM_001901 CTGF Connective tissue growth factor
B03 NM_003467 CXCR4 Chemokine (C-X-C motif) receptor 4
B04 NM_001920 DCN Decorin
B05 NM_001955 EDN1 Endothelin 1
B06 NM_001963 EGF Epidermal growth factor
B07 NM_000118 ENG Endoglin
B08 NM_000639 FASLG Fas ligand (TNF superfamily, member 6)
B09 NM_013372 GREM1 Gremlin 1
B10 NM_000601 HGF Hepatocyte growth factor (hepapoietin A; scatter factor)
B11 NM_000619 IFNG Interferon, gamma
B12 NM_000572 IL10 Interleukin 10
C01 NM_001844 COL2a1 Collagen, type II, alpha 1
C02 NM_001845 COL4A1 Collagen, type IV, alpha 1
C03 NM_001846 COL4A2 Collagen, type IV, alpha 2
C04 NM_000093 COL5A1 Collagen, type V, alpha 1
C05 NM_000393 COL5A2 Collagen, type V, alpha 2
C06 NM_030582 COL18A1 Collagen, type XVIII, alpha 1
C07 NM_002188 IL13 Interleukin 13
C08 NM_000640 IL13RA2 Interleukin 13 receptor, alpha 2
C09 NM_000575 IL1A Interleukin 1, alpha
C10 NM_000576 IL1B Interleukin 1, beta
C11 NM_004517 ILK Integrin-linked kinase
C12 NM_031479 INHBE inhibin, beta E
D01 NM_001800 CDKN2D Cyclin-dependent kinase inhibitor 2D 
D02 NM_058229 FBXo32 F-box protein 32
D03   PTGS2 (cox-2) Prostaglandin-endoperoxide synthase 2
D04 NM_022787 NMNAT1 Nicotinamide nucleotide adenylyltransferase 1
D05 NM_000492 CFTR Cystic fibrosis transmembrane conductance regulator
D06 NM_002228 JUN Jun proto-oncogene
D07 NM_002317 LOX Lysyl oxidase
D08 NM_000627 LTBP1 Latent transforming growth factor beta binding protein 1
D09 NM_002421 MMP1 Matrix metallopeptidase 1 (interstitial collagenase)
D10 NM_002427 MMP13 Matrix metallopeptidase 13 (collagenase 3)
D11 NM_004995 MMP14 Matrix metallopeptidase 14 (membrane-inserted)
D12 NM_004530 MMP2 Matrix metallopeptidase 2 (72kDa type IV collagenase)
E01 NM_002422 MMP3 Matrix metallopeptidase 3 (stromelysin 1, progelatinase)
E02 NM_002424 MMP8 Matrix metallopeptidase 8 (neutrophil collagenase)
E03 NM_004994 MMP9 Matrix metallopeptidase 9 (92kDa type IV collagenase)
E04 NM_003106 Sox2 SRY (sex determining region Y)-box 2
E05 NM_003998 NFKB1 Nuclear factor of kappa light polypeptide gene enhancer in B-cells 1
E06 NM_002607 PDGFA Platelet-derived growth factor alpha polypeptide
E07 NM_002608 PDGFB Platelet-derived growth factor beta polypeptide
E08 NM_000930 PLAT Plasminogen activator, tissue
E09 NM_002658 PLAU Plasminogen activator, urokinase
E10 NM_000301 PLG Plasminogen
E11 NM_000295 SERPINA1 Alpha-1 antiproteinase
E12 NM_000602 SERPINE1 Plasminogen activator inhibitor type 1
F01 NM_001235 SERPINH1 Heat shock protein 47
F02 NM_005901 SMAD2 SMAD family member 2
F03 NM_005902 SMAD3 SMAD family member 3
F04 NM_005359 SMAD4 SMAD family member 4
F05 NM_005585 SMAD6 SMAD family member 6
F06 NM_005904 SMAD7 SMAD family member 7
F07 NM_005985 SNAI1 Snail homolog 1 (Drosophila)
F08 NM_002961.2 S100A4 S100 calcium binding protein A4
F09 NM_007315 STAT1 Signal transducer and activator of transcription 1, 91kDa
F10 NM_003153 STAT6 Signal transducer and activator of transcription 6
F11 NM_000660 TGFB1 Transforming growth factor, beta 1
F12 NM_003238 TGFB2 Transforming growth factor, beta 2
G01 NM_003239 TGFB3 Transforming growth factor, beta 3
G02 NM_004612 TGFBR1 Transforming growth factor, beta receptor 1
G03 NM_003242 TGFBR2 Transforming growth factor, beta receptor II (70/80kDa)
G04 NM_003244 TGIF1 TGFB-induced factor homeobox 1
G05 NM_003246 THBS1 Thrombospondin 1
G06 NM_003247 THBS2 Thrombospondin 2
G07 NM_003254 TIMP1 TIMP metallopeptidase inhibitor 1
G08 NM_003255 TIMP2 TIMP metallopeptidase inhibitor 2
G09 NM_000362 TIMP3 TIMP metallopeptidase inhibitor 3
G10 NM_003256 TIMP4 TIMP metallopeptidase inhibitor 4
G11 NM_000594 TNF Tumor necrosis factor
G12 NM_003376 VEGFA Vascular endothelial growth factor A
H01 NM_004048 B2M Beta-2-microglobulin
H02 NM_138712 Ppar g Peroxisome proliferator-activated receptor gamma 
H03 NM_012423 RPL13A Ribosomal protein L13a
H04 NM_006288 Thy1 Thy-1 cell surface antigen
H05 NM_003018 SFTPC Surfactant protein C 
H06 NM_003529 HIST1H3A Histone cluster 1, H3a
H07 NM_002046 GAPDH Glyceraldehyde-3-phosphate dehydrogenase
H08 NM_002046 GAPDH Glyceraldehyde-3-phosphate dehydrogenase
H09 NM_001101 ACTB Actin, beta
H10 NM_001101 ACTB Actin, beta
H11 NM_006082 Tuba1b Homo sapiens tubulin, alpha 1b (TUBA1B)
H12 NM_000194 HPRT1 Hypoxanthine phosphoribosyltransferase 1

PCR Array Layout (96-Well)