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Human Th1-Th2-th17 qPCR Array
2 or 12 plates plus qPCR MasterMix
Price: From $199.00 to $1,199.00

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Detailed Description

This Th1-Th2-Th17 Array is designed to profile the expression of 91 key genes related to the three classes of helper T cells (CD4+ T lymphocytes): Th1, Th2, and Th17.

Features

  • More genes featured for each pathway
  • Fits to ABI, BioRad and many other Real-Time thermocyclers
  • Strong detection signals without leaking to the adjacent wells
  • Higher sensitivity and sharper melting curve
  • All sets of primers span at least one intron of the genomic sequence with annealing temperatures of 60 ± 1 oC
  • EvaGreen PCR MasterMix (2x) included

Catalog Number

qPCR Array Format

Real-Time Thermal Cyclers

GA-T104A

Human Th1-Th2-Th17 qPCR Array Format A

Bio-Rad CFX96, CFX384; Bio-Rad/MJ Research models Chrom4, DNA Engine Bio-Rad models iCycler, iQ5, MyiQ, MyiQ2, Opticon 2; Roche LightCycler 480 (96).

GA-T104B

Human Th1-Th2-Th17 qPCR Array Format B

Applied Biosystems models 5700, 7300, 7500 (Standard and Fast), 7700, 7900HT (Standard and Fast); StepOnePlus, ViiA7 (Standard and Fast); Eppendorf Mastercycler ep realplex models 2, 2S, 4, 4S; Stratagene models Mx3000P, Mx3005P, Mx4000; Takara TP-800.

Gene Information

Position

GeneBank

Symbol

Name

A01

NM_002190

IL17A

Interleukin 17A

A02

NM_002986

CCL11

Chemokine (C-C motif) ligand 11

A03

NM_002985

CCL5

Chemokine (C-C motif) ligand 5

A04

NM_006273

CCL7

Chemokine (C-C motif) ligand 7

A05

NM_001123396

CCR2

Chemokine (C-C motif) receptor 2

A06

NM_001837

CCR3

Chemokine (C-C motif) receptor 3

A07

NM_005508

CCR4

Chemokine (C-C motif) receptor 4

A08

NM_000579

CCR5

Chemokine (C-C motif) receptor 5

A09

NM_006139

CD28

CD28 molecule

A10

NM_000616

CD4

CD4 molecule

A11

NM_000074

CD40LG

CD40 ligand

A12

NM_171827.3

CD8a

 CD8a molecule

B01

NM_005755.2

EBI3

Epstein-Barr virus induced 3

B02

NM_016584

IL23A

Interleukin 23, alpha subunit p19

B03

NM_144701.2

IL23R

Interleukin 23 receptor

B04

NM_145659.3

IL27

Interleukin 27

B05

NM_004843.2

IL27RA

Interleukin 27 receptor alpha

B06

NM_005191

CD80

CD80 molecule

B07

NM_006889

CD86

CD86 molecule

B08

NM_005194

CEBPB

CCAAT/enhancer binding protein (C/EBP), beta

B09

NM_004380

CREBBP

CREB binding protein

B10

NM_000758

CSF2

Colony stimulating factor 2 (granulocyte-macrophage)

B11

NM_005214

CTLA4

Cytotoxic T-lymphocyte-associated protein 4

B12

NM_001504

CXCR3

Chemokine (C-X-C motif) receptor 3

C01

NM_000639

FASLG

Fas ligand (TNF superfamily, member 6)

C02

NM_014009.3

Foxp3

Forkhead box P3

C03

NM_002051

GATA3

GATA binding protein 3

C04

NM_005263

GFI1

Growth factor independent 1 transcription repressor

C05

NM_053274

GLMN

Glomulin, FKBP associated protein

C06

NM_004778

GPR44

G protein-coupled receptor 44

C07

NM_032782

HAVCR2

Hepatitis A virus cellular receptor 2

C08

NM_012092

ICOS

Inducible T-cell co-stimulator

C09

NM_000619

IFNG

Interferon, gamma

C10

NM_005849

IGSF6

Immunoglobulin superfamily, member 6

C11

NM_000572

IL10

Interleukin 10

C12

NM_002187

IL12B

Interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40)

D01

NM_001559

IL12RB2

Interleukin 12 receptor, beta 2

D02

NM_002188

IL13

Interleukin 13

D03

NM_001560

IL13RA1

Interleukin 13 receptor, alpha 1

D04

NM_000585

IL15

Interleukin 15

D05

NM_001562

IL18

Interleukin 18 (interferon-gamma-inducing factor)

D06

NM_003855

IL18R1

Interleukin 18 receptor 1

D07

NM_000877

IL1R1

Interleukin 1 receptor, type I

D08

NM_004633

IL1R2

Interleukin 1 receptor, type II

D09

NM_000586

IL2

Interleukin 2

D10

NM_000417

IL2RA

Interleukin 2 receptor, alpha

D11

NM_000589

IL4

Interleukin 4

D12

NM_000418

IL4R

Interleukin 4 receptor

E01

NM_000879

IL5

Interleukin 5 (colony-stimulating factor, eosinophil)

E02

NM_000600

IL6

Interleukin 6 (interferon, beta 2)

E03

NM_000565

IL6R

Interleukin 6 receptor

E04

NM_000880

IL7

Interleukin 7

E05

NM_000590

IL9

Interleukin 9

E06

NM_002191

INHA

Inhibin, alpha

E07

NM_002192

INHBA

Inhibin, beta A

E08

NM_002198

IRF1

Interferon regulatory factor 1

E09

NM_002460

IRF4

Interferon regulatory factor 4

E10

NM_002227

JAK1

Janus kinase 1

E11

NM_004972

JAK2

Janus kinase 2

E12

NM_000215 

JAK3

Janus kinase 3

F01

NM_002286

LAG3

Lymphocyte-activation gene 3

F02

NM_014387

LAT

Linker for activation of T cells

F03

NM_005360

MAF

V-maf musculoaponeurotic fibrosarcoma oncogene homolog (avian)

F04

NM_145185

MAP2K7

Mitogen-activated protein kinase kinase 7

F05

NM_002750

MAPK8

Mitogen-activated protein kinase 8

F06

NM_172390

NFATC1

Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 1

F07

NM_012340

NFATC2

Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2

F08

NM_032815

NFATC2IP

Nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2 interacting protein

F09

NM_007144

PCGF2

Polycomb group ring finger 2

F10

NM_002838

PTPRC

Protein tyrosine phosphatase, receptor type, C

F11

NM_005060.3

RORC

Homo sapiens RAR-related orphan receptor C

F12

NM_003019

SFTPD

Surfactant protein D

G01

NM_003745

SOCS1

Suppressor of cytokine signaling 1

G02

NM_003877

SOCS2

Suppressor of cytokine signaling 2

G03

NM_144949

SOCS5

Suppressor of cytokine signaling 5

G04

NM_000582

SPP1

Secreted phosphoprotein 1

G05

NM_007315

STAT1

Signal transducer and activator of transcription 1, 91kDa

G06

NM_003151

STAT4

Signal transducer and activator of transcription 4

G07

NM_003153

STAT6

Signal transducer and activator of transcription 6, interleukin-4 induced

G08

NM_013351

TBX21

T-box 21

G09

NM_005653

TFCP2

Transcription factor CP2

G10

NM_003239

TGFB3

Transforming growth factor, beta 3

G11

NM_138554

TLR4

Toll-like receptor 4

G12

NM_006068

TLR6

Toll-like receptor 6

H01

NM_006858

TMED1

Transmembrane emp24 protein transport domain containing 1

H02

NM_000594

TNF

Tumor necrosis factor

H03

NM_001242

CD27

CD27 molecule

H04

NM_001243

TNFRSF8

Tumor necrosis factor receptor superfamily, member 8

H05

NM_001561

TNFRSF9

Tumor necrosis factor receptor superfamily, member 9

H06

NM_003326

TNFSF4

Tumor necrosis factor (ligand) superfamily, member 4

H07

NM_003331

TYK2

Tyrosine kinase 2

H08

NM_002046

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

H09

NM_002046

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

H10

NM_001101

ACTB

Actin, beta

H11

NM_006082.2

TUBA1B

Tubulin, alpha 1b

H12

NM_000194

HPRT1

Hypoxanthine phosphoribosyltransferase 1



PCR Array Layout (96-Well)