MaxPrep^TM Transfection Reagent is a non-liposomal transfection reagent formulated for high yield of protein production in mammalian cells.

Features

• High efficiency but low toxicity in various cell types

• MaxPrep^TM-DNA complex can be added directly to cells in culture medium, in the presence of antibiotics and serum

• Slow release formulation guaranteed the low toxicity and high transfection efficiency during the incubation

• Eliminates the need for media changes after transfection

• Excellent for industry level protein expression such as in HEK293T, 293F, CHO and 293S cells

• Alternative and affordable transfection reagent for large scale protein production, reduce your cost up to 50% compare to Invitrogen's 293Fectin^TM

Applications

• Transient and stable transfection in mammalian cells

• Highly efficient transfection reagent in all cell types

• Large scale protein production in mammalian cells

Size

1ml (>300 transfections in 12-well plate).

Experimental Data:

Figure 1. Comparison study of transfection efficiency of MaxPrep^TM with other brand name transfection reagents.  3 µl of transfection reagent is combined with 1 µg pg GFP reporter gene DNA, and used to transfect 293 cells in a 12-well plate. 5 and 6, MaxPrep^TM transfection reagent; 1, PEI 25K; 2, Brand A transfection reagent; 3 and 7, Brand B lipid-based transfection reagent; 4, Brand C transfection reagent; 8, Brand D transfection reagent.

Figure 2. FACS assay of MaxPrep^TM and lipid-based Brand B transfection reagent on 293F cell. A, B, C, plasmid DNA/Brand B transfection reagent=1:3; D, E, F,  plasmid DNA/MaxPrep^TM transfection reagent = 1:3. A & D, 1 µg DNA transfected; B &E, 2 µg DNA transfected; C & F, 3 µg DNA transfected.

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