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Animal-Free Recombinant Human Basic Fibroblast Growth Factor (r-bFGF)

Catalog Number:
HbFGF-50r
Price: $125.00
Detailed Description


Human basic Fibroblast Growth Factor (bFGF) is a single-chain polypeptide growth factor that plays a significant role in the process of wound healing and is a potent inducer of angiogenesis. bFGF has been shown to support the maintenance of undifferentiated human embryonic stem cells. It also stimulates the proliferation of all cells of mesodermal origin, and many cells of neuroectodermal, ectodermal and endodermal origin. bFGF is a chemotactic and mitogenic agent for endothelial cells in vitro and induces neural differentiation, survival and regeneration. It has been shown to be crucial in modulation of angiogenesis, tissue repair, embryonic development and neuronal function in vivo.

PRODUCT DESCRIPTION

r-bFGF is a human recombinant bFGF protein produced in Rice, which is a single non-glycosylated polypeptide chain containing 146 amino acids and has a molecular weight of 17 kD. The r-bFGF protein is purified by chromatographic techniques.

Application

Human embryonic stem cell and various other stem cell line such as mesenchymal stem (stroma) cell (MSC) culture

SOURCE

Rice Grain (Oryza Sativa)

Size

50 µg

FORMULATION

Lyophilized powder containing sodium phosphate and sodium chloride, pH6.5-7.5

STORAGE TEMPERATURE

-20°C

ENDOTOXIN

≤1 EU/µg

PURITY

>95% by SDS-PAGE

ACTIVITY

The ED50 calculated by the dose-dependent proliferation of Balb/c 3T3 cells expressing FGF receptors is 1 ng/ml, corresponding to a specific activity of (1 x106) Units/mg.


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