5-Hydroxytryptamin Receptor 2C (HTR2C)

Name

5-Hydroxytryptamin (serotonin) receptor 2C (HTR2C) stable cell line

Description

This stable cell line expresses a recombinant human HTR2C. This tetracycline inducible cell line carries a double twin-strep-tag at the N-terminus and a 10-His tag at the C-terminus. There are two TEV cleavage sites inserted between the tags and the receptor for tags' removal.

For all membrane preparations for which radio ligand binding characterization has been conducted, we deliver 1μg/unit, as soon as 1μg is enough to obtain 1000 specific DPM at the Kd concentration of the radio ligand of reference.

For all membrane preparations for which radio ligand binding characterization has not yet been conducted, we deliver 10μg/unit.

Characterizations

High level receptor expression (over 1 million receptors per cell);

Tetracycline inducible and well suited for 13C, 15N labeling;

Removable tags allowing purification by streptactin and/or nickel/cobalt column.

Size