Adenosine A2A Receptor (ADORA2A) ACTOne Stable Cell Line
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Catalog Number:
CL-01-ADORA2A
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In Stock
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Price: Please inquire order@eEnzyme.com
Detailed Description
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CL-01-ADORA2A
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Name
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Adenosine A2A Receptor (ADORA2A) ACTOne™ Stable Cell Line
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Description
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This cell line is a HEK-293-CNG cell line that expresses a recombinant human ADORA2A. HEK-293-CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux and cell membrane depolarization.
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Application
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cAMP dependent human ADORA2A cell-based assay;
cell-based high-throughput screening of human ADORA2A agonists/antagonists.
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Size
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1x 106 cells
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Detection
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FlexStation or Microplate reader
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For Downloading
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==>> DATA SHEET ==>> MSDS
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About Human Adenosine A2A Receptor (ADORA2A/A2AR) Stable Cell Line
Adenosine A2A Receptor (ADORA2A), also known as A2A receptor (A2AR), is a Class A G protein-coupled receptor (GPCR) that is activated by the endogenous nucleoside adenosine. ADORA2A primarily couples to Gs proteins, stimulating adenylate cyclase activity and increasing intracellular cAMP levels. The receptor is highly expressed in the basal ganglia of the brain, as well as in immune cells, platelets, vascular endothelial cells, and the cardiovascular system, where it regulates neurotransmission, inflammation, vasodilation, and immune responses.
ADORA2A plays critical roles in dopaminergic neurotransmission, neuroprotection, and inflammatory regulation. It has become an important therapeutic target for Parkinson's disease, neurodegenerative disorders, cancer immunotherapy, cardiovascular diseases, and chronic inflammatory conditions. In addition, caffeine is one of the best-known competitive antagonists of the A2A receptor, making ADORA2A one of the most extensively studied purinergic GPCRs.
eEnzyme's Human ADORA2A Stable Cell Line provides a reliable platform for investigating A2A receptor-mediated signaling and evaluating agonists, antagonists, and novel receptor modulators using cAMP and other GPCR functional assays. The cell line is well suited for receptor characterization, compound profiling, potency determination, and high-throughput drug screening.
Applications
• ADORA2A (A2AR) receptor pharmacology studies
• Adenosine signaling research
• Agonist and antagonist screening
• Parkinson's disease research
• Cancer immunotherapy research
• Inflammation and immunology studies
• cAMP and GPCR functional assays
• High-throughput screening (HTS)
Biological Significance of ADORA2A
ADORA2A is a key mediator of adenosine signaling and regulates numerous physiological processes through Gs-mediated cAMP signaling. In the central nervous system, the receptor modulates dopamine and glutamate neurotransmission, influencing motor control, cognition, sleep, and reward pathways. In peripheral tissues, ADORA2A suppresses excessive inflammatory responses, promotes vasodilation, and regulates platelet and immune cell function. Because of its broad roles in neurological, cardiovascular, inflammatory, and immune disorders, ADORA2A has become an important therapeutic target for Parkinson's disease, cancer immunotherapy, chronic inflammation, and autoimmune diseases. The ADORA2A Stable Cell Line provides a valuable tool for studying adenosine receptor biology and accelerating the discovery of next-generation therapeutics targeting A2A receptor signaling.
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