About Human Corticotropin-Releasing Hormone Receptor 2 (CRHR2/CRF2) Stable Cell Line

Corticotropin-Releasing Hormone Receptor 2 (CRHR2), also known as CRF2 receptor, is a Class B G protein-coupled receptor (GPCR) that belongs to the corticotropin-releasing hormone receptor family. CRHR2 is activated primarily by members of the urocortin peptide family, including urocortin 1, urocortin 2, and urocortin 3, and exhibits lower affinity for corticotropin-releasing hormone (CRH) than CRHR1. The receptor is expressed in multiple tissues, including the brain, heart, skeletal muscle, gastrointestinal tract, vasculature, and endocrine organs. Upon ligand binding, CRHR2 primarily couples to Gs proteins, leading to activation of adenylate cyclase, increased intracellular cAMP production, and downstream signaling pathways that regulate stress adaptation, cardiovascular function, metabolism, and neuroendocrine responses.

CRHR2 serves important functions in modulating and counterbalancing stress responses initiated through CRHR1 signaling. While CRHR1 is generally associated with activation of the hypothalamic-pituitary-adrenal (HPA) axis during acute stress, CRHR2 contributes to stress recovery, emotional regulation, cardiovascular homeostasis, feeding behavior, and energy metabolism. Dysregulation of CRHR2 signaling has been implicated in anxiety disorders, depression, cardiovascular disease, obesity, metabolic dysfunction, inflammatory disorders, and gastrointestinal diseases. Consequently, CRHR2 has emerged as an important therapeutic target for neuroscience, cardiometabolic research, and stress-related disease drug discovery.

eEnzyme's Human CRHR2 Stable Cell Line provides a reliable and reproducible cellular platform for investigating CRHR2-mediated signaling pathways and evaluating receptor-targeted therapeutics. The cell line enables quantitative assessment of urocortins, CRH analogs, agonists, antagonists, and novel receptor modulators through cAMP assays, reporter gene assays, and other GPCR functional screening platforms. Researchers can utilize this stable cell line for receptor characterization, ligand profiling, potency determination, lead optimization, and high-throughput screening applications supporting neuroscience, cardiovascular, metabolic, and endocrine research programs.

Applications of the CRHR2 Stable Cell Line

• CRHR2 receptor pharmacology studies
• Urocortin signaling investigations
• Agonist and antagonist screening
• Stress adaptation and resilience research
• Anxiety and depression studies
• Cardiovascular and metabolic disease research
• cAMP and GPCR functional assays
• High-throughput screening applications

Biological Significance of CRHR2

CRHR2 plays a critical role in maintaining physiological balance during and after stress exposure. Activation of the receptor by urocortin peptides helps regulate cardiovascular function, vascular tone, feeding behavior, energy expenditure, and emotional responses. In the central nervous system, CRHR2 signaling contributes to stress coping mechanisms and may exert anxiolytic effects under certain physiological conditions. In peripheral tissues, CRHR2 regulates cardiac performance, blood vessel function, glucose metabolism, and gastrointestinal activity. Growing evidence suggests that impaired CRHR2 signaling may contribute to stress-related psychiatric disorders, metabolic syndrome, obesity, and cardiovascular disease. Because of its unique role in stress adaptation and systemic homeostasis, CRHR2 remains an important target for translational research and GPCR-focused drug discovery. The CRHR2 Stable Cell Line provides a valuable tool for studying receptor biology and advancing the development of next-generation therapeutics targeting neuropsychiatric, cardiovascular, and metabolic disorders.