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High Fidelity DNA Polymerase

Catalog Number:
DP-003-0250
Price: $119.00
Detailed Description

DP-003-0250

Description

Elite™ High Fidelity DNA Polymerase is a thermostable enzyme possessing 5'-3' DNA polymerase and 3'-5' proofreading exonuclease activities. It is isolated from the hyperthermophilic marine archae Pyrococcus furiosis (Pfu). Base mis-insertions that may occur during polymerization are rapidly excised by the proofreading activity of the polymerase. Elite™ DNA Polymerase could amplify cDNA up to 5kb. It generates blunt-ended PCR fragments.

Application

Designed for extremely high fidelity PCR applications, such as subcloning of PCR amplified cDNA or cDNA library construction.

Concentration

5 units/µl

Storage

-20 oC

Size

250 Units

For Downloading

===> DATA SHEET


Selected Citation:

Millington, A. L., Houskeeper, J. A., Quackenbush, J. F., Trauba, J. M. & Wittwer, C. T. The kinetic requirements of extreme qPCR. Biomol. Detect. Quantif. 17, 100081 (2019).