Melanocortin 5 Receptor (MC5R) ACTOne Stable Cell Line
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Catalog Number:
CL-01-MC5R
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In Stock
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Price: Please inquire order@eEnzyme.com
Detailed Description
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CL-01-MC5R
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Name
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Melanocortin 5 Receptor (MC5R) ACTOneTM Stable Cell Line
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Description
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This cell line is a HEK-293-CNG cell line that expresses a recombinant human MC5R. HEK-293-CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux and cell membrane depolarization. The assay allows both end-point and kinetic measurement of intracellular cAMP changes with a FLIPR or a fluorescence microplate reader.
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Application
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cAMP dependent human MC5R cell-based assay;
Cell-based high-throughput screening of human MC5R agonists/antagonists.
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Size
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1x 106 cells
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Detection
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FlexStation or Microplate reader
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For Downloading
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==>> DATA SHEET ==>> MSDS
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Synonym: Melanocortin 5 Receptor, Melanocortin Receptor 5, MC5-R, MC-2, MC2
About Human Melanocortin 5 Receptor (MC5R) Stable Cell Line
Melanocortin 5 Receptor (MC5R) is a Class A G protein-coupled receptor (GPCR) that is activated by endogenous melanocortin peptides, including α-melanocyte-stimulating hormone (α-MSH), β-MSH, γ-MSH, and adrenocorticotropic hormone (ACTH), which are derived from the proopiomelanocortin (POMC) precursor. MC5R primarily couples to Gs proteins, stimulating adenylate cyclase activity and increasing intracellular cAMP levels. Unlike other melanocortin receptors, MC5R is widely expressed in both the central nervous system and peripheral tissues, including sebaceous glands, exocrine glands, skeletal muscle, adipose tissue, immune cells, and skin, where it regulates exocrine secretion, energy metabolism, lipid homeostasis, and immune function.
MC5R plays important roles in sebaceous gland function, thermoregulation, lipid metabolism, skeletal muscle physiology, and inflammatory responses. Dysregulation of MC5R signaling has been associated with metabolic disorders, inflammatory diseases, skin disorders, and impaired exocrine gland function, making the receptor an emerging therapeutic target for metabolic and inflammatory disease research.
eEnzyme's Human MC5R Stable Cell Line provides a reliable platform for investigating MC5R-mediated signaling and evaluating agonists, antagonists, and novel receptor modulators using cAMP and other GPCR functional assays. The cell line is well suited for receptor characterization, compound profiling, potency determination, and high-throughput drug screening.
Applications
• MC5R receptor pharmacology studies
• Melanocortin signaling research
• Agonist and antagonist screening
• Metabolic disease research
• Lipid metabolism and sebaceous gland studies
• Inflammation and immunology research
• cAMP and GPCR functional assays
• High-throughput screening (HTS)
Biological Significance of MC5R
MC5R is the most widely distributed member of the melanocortin receptor family and regulates diverse physiological processes beyond energy homeostasis. Activation of the receptor stimulates Gs-mediated cAMP signaling, influencing exocrine gland secretion, lipid metabolism, skeletal muscle energy utilization, thermoregulation, and immune responses. In contrast to MC3R and MC4R, which primarily regulate appetite and body weight, MC5R plays a prominent role in peripheral tissue function, particularly in sebaceous glands and metabolic tissues. Growing evidence suggests that selective modulation of MC5R may provide therapeutic opportunities for inflammatory diseases, metabolic disorders, skin diseases, and tissue protection. The MC5R Stable Cell Line provides a valuable tool for studying melanocortin receptor biology and accelerating the development of next-generation therapeutics targeting metabolic and inflammatory pathways.
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