MERS-CoV Pseudoviral Particles
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Catalog Number:
MERS-PsV-001
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MERS-CoV Pseudoviral Particles, replication-deficient MLV pseudotyped with the MERS spike protein. (Mobile users please click "View Full Site" for details)
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Detailed Description
Keywords: coronavirus, SARS, SARS-Cov, SARS-COV-2, covid19, covid-19, SARS pseudovirus, sars-cov-2 pseudovirus, sars-cov-2 spike, ace2, SARS-2, new SARS, SARS-Cov-2, nCOV2019, pseudovirus, pseudoviral particle, spike, MLV, LUC
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MERS-PsV-001
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Name
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MERS-CoV Pseudoviral Particles
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Description
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The MERS Pseudoviral Particles we produce are replication-deficient Maloneymurine Leukemia Virus (MLV or MuLV) pseudotyped with the MERS virus spike protein (Genbank Accession # AHY21469.1).
MLV-based virus system is a better choice than HIV-based lentivirus system for making pseudoviral particles in regards of lab safety and product quality.
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Applications
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Our Pseudovirus Particles generate robust chemiluminescent signals in cell assays when coupled with our firefly luciferase assay kit (Catalog # CA-L165), useful for 1) screening potential inhibitors to block MERS virus entry and viral protein translation with spike protein of MERS; 2) measuring the activity of and screening for neutralizing antibody against MERS virus.
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Size
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5 ml, for one multi-well plates. Pseudoviral particles per mL ~ 1.0E+08
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Storage
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-70 C. Shipped on dry ice.
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Note
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Amenable to HTS format (96-well, 384-well and 1536-well format).
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For Downloading
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==>> DATA SHEET ==>> SAFETY DATA SHEET |
About MERS-CoV Pseudovirus Particles
Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is an enveloped, positive-sense single-stranded RNA virus belonging to the genus Betacoronavirus. Viral entry is mediated by the Spike (S) glycoprotein, which binds the cellular receptor DPP4/CD26 and subsequently promotes membrane fusion with host cells. Because the spike protein determines viral tropism and is the primary target of neutralizing antibodies, it is widely used in studies of viral entry, receptor binding, and antiviral development.
eEnzyme's MERS-CoV Pseudovirus Particles are replication-deficient viral particles that express the MERS-CoV spike protein on their surface while carrying a reporter gene for quantitative detection of infection. These pseudoviral particles provide a safe and convenient BSL-2-compatible platform for investigating spike-mediated viral entry, DPP4 receptor interactions, neutralizing antibody activity, antiviral compound screening, and vaccine evaluation without the need to handle infectious MERS-CoV. Pseudovirus-based assays have become widely adopted for coronavirus research because they closely mimic viral entry while offering excellent reproducibility and laboratory safety.
Applications
• Neutralizing antibody assays
• Antiviral compound screening
• MERS-CoV vaccine evaluation
• DPP4/CD26 receptor-binding studies
• Spike-mediated viral entry research
• Viral tropism and host-cell interaction studies
• High-throughput screening (HTS)
• Coronavirus research
Biological Significance of MERS-CoV Pseudovirus Particles
MERS-CoV pseudovirus particles faithfully reproduce the spike-dependent entry process of authentic MERS-CoV while remaining replication incompetent. They provide a robust experimental system for investigating virus-host interactions, evaluating neutralizing antibodies, characterizing spike variants, and screening antiviral therapeutics under standard BSL-2 laboratory conditions. Consequently, MERS pseudovirus assays have become an essential tool for coronavirus research, vaccine development, and therapeutic discovery.
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