About Human Neuropeptide Y Receptor Y2 (NPY2R/Y2 Receptor) Stable Cell Line

Neuropeptide Y Receptor Y2 (NPY2R), also known as the Y2 receptor, is a Class A G protein-coupled receptor (GPCR) belonging to the neuropeptide Y (NPY) receptor family. NPY2R is activated by endogenous ligands including neuropeptide Y (NPY), peptide YY (PYY), and NPY fragments such as NPY(3-36). Upon ligand binding, NPY2R primarily couples to Gi/o proteins, resulting in inhibition of adenylate cyclase activity, decreased intracellular cAMP levels, modulation of ion channel activity, and regulation of downstream signaling pathways involved in appetite control, energy homeostasis, neurotransmission, and endocrine function.

NPY2R is widely expressed in the central nervous system and peripheral tissues, including the hypothalamus, brainstem, gastrointestinal tract, pancreas, and adipose tissue. The receptor plays a critical role in regulating feeding behavior, satiety, body weight maintenance, stress responses, anxiety, and neuroendocrine signaling. In particular, activation of Y2 receptors by peptide YY released from the gastrointestinal tract after food intake contributes to appetite suppression and energy balance. Because of its involvement in obesity, metabolic disorders, diabetes, and central nervous system regulation, NPY2R has emerged as an important therapeutic target for drug discovery and pharmacological research.

eEnzyme's Human NPY2R Stable Cell Line provides a reliable and reproducible cellular platform for investigating Y2 receptor pharmacology and signaling mechanisms. The cell line enables quantitative assessment of agonists, antagonists, inverse agonists, and allosteric modulators through cAMP assays, reporter gene assays, and other GPCR functional screening platforms. Researchers can utilize this stable cell line for receptor characterization, compound profiling, lead optimization, and high-throughput screening applications targeting metabolic, gastrointestinal, and neurological diseases.

Applications of the NPY2R Stable Cell Line

• NPY2R receptor pharmacology studies
• Peptide YY (PYY) and neuropeptide Y signaling investigations
• Agonist and antagonist screening
• Appetite regulation and obesity research
• Metabolic disease drug discovery programs
• cAMP and GPCR signaling assays
• Neuroendocrine and neuroscience research
• High-throughput screening applications

Biological Significance of NPY2R

NPY2R functions as an important regulator of appetite, energy balance, and neuronal signaling. Activation of Y2 receptors inhibits neurotransmitter release through presynaptic mechanisms and contributes to the regulation of feeding behavior and satiety. In the gastrointestinal system, PYY-mediated activation of NPY2R helps communicate nutritional status to the brain, reducing food intake and supporting body weight regulation. Beyond metabolic control, NPY2R also participates in stress responses, anxiety regulation, cardiovascular function, and neuroprotection. Because of its central role in appetite suppression and energy homeostasis, NPY2R remains an attractive target for the development of therapies for obesity, metabolic syndrome, diabetes, and other disorders associated with dysregulated energy balance. The NPY2R Stable Cell Line provides a valuable tool for investigating receptor biology and advancing GPCR-targeted drug discovery programs.