Neuraminidase Assay Kit (Blue Fluorescence)
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Catalog Number:
CA-N602
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For quantifying neuraminidase activity
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Detailed Description
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CA-N602
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Name
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Elite™ Neuraminidase Assay Kit
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Description
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This neuraminidase assay kit provides a sensitive and robust fluorimetric assay to detect neuraminidase that exists in either cells or other biological samples.
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Application
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The assay is designed for the detection of influenza neuraminidase and other viral / non-viral neuraminidase activity in a variety of biological fluid samples and cells.
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Size
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200 assays (or two 96-well plates)
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Detection
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Fluorescence microplate reader
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For Downloading
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==>> DATA SHEET ==>> MSDS
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About Elite™ Neuraminidase Assay Kit (Blue Fluorescence)
Neuraminidase (NA), also known as sialidase (EC 3.2.1.18), is an enzyme that cleaves terminal sialic acid residues from glycoproteins and glycolipids. Viral neuraminidases, particularly those from influenza A and B viruses, play an essential role in the viral life cycle by facilitating the release of newly formed virions from infected cells and promoting viral spread. Neuraminidases are also produced by numerous bacteria and mammalian cells, where they participate in cell signaling, host-pathogen interactions, immune regulation, and glycoconjugate metabolism. Because of their important biological functions, neuraminidases have become valuable targets for antiviral drug development, infectious disease research, and enzyme inhibitor screening.
eEnzyme's Elite™ Neuraminidase Assay Kit (Blue Fluorescence) provides a sensitive and convenient fluorometric assay for quantifying neuraminidase activity in cultured cells, biological fluids, tissue extracts, purified enzymes, and viral samples. The assay utilizes a non-fluorescent substrate that releases a highly fluorescent product following neuraminidase-mediated cleavage, enabling rapid and accurate measurement of enzyme activity. The simple mix-and-read format is compatible with both 96- and 384-well microplates, making the kit well suited for routine laboratory analysis as well as high-throughput screening of neuraminidase inhibitors.
Applications
• Influenza neuraminidase activity measurement
• Viral infection and replication studies
• Neuraminidase inhibitor screening
• Antiviral drug discovery
• Sialidase activity quantification
• Enzyme kinetics and inhibition studies
• High-throughput compound screening
• Glycobiology and infectious disease research
Biological Significance of Neuraminidase
Neuraminidases regulate the removal of terminal sialic acid residues from glycoconjugates, influencing cell-cell interactions, receptor accessibility, immune responses, and pathogen infectivity. Influenza viruses depend on neuraminidase activity to efficiently release progeny virions and spread between host cells, making the enzyme a primary target of antiviral drugs such as oseltamivir, zanamivir, peramivir, and laninamivir. Beyond virology, neuraminidases contribute to bacterial pathogenesis, lysosomal metabolism, and cellular signaling pathways. Sensitive measurement of neuraminidase activity is therefore essential for studies of viral biology, enzyme function, therapeutic inhibitor development, and biomedical research.
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