CL-01-PTGER2
Name	Prostaglandin E2 Receptor ACTOneTM Stable Cell Line
Description	This cell line is a HEK-293-CNG cell line that expresses a recombinant human PTGER2. HEK-293-CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux and cell membrane depolarization. The assay allows both end-point and kinetic measurement of intracellular cAMP changes with a FLIPR or a fluorescence microplate reader.
Application	cAMP dependent human PTGER2 cell-based assay; Cell-based high-throughput screening of human PTGER2 agonists/antagonists.
Size	1x 106 cells
Detection	FlexStation or Microplate reader
For Downloading	==>> DATA SHEET      ==>> MSDS

Synonym: Prostaglandin E Receptor 2, EP2, Prostaglandin E Receptor 2 (Subtype EP2), Prostaglandin E Receptor 2 (Subtype EP2), Prostaglandin E2 Receptor EP2 Subtype, PGE2 Receptor EP2 Subtype, PGE Receptor EP2 Subtype, Prostanoid EP2 Receptor

About Human Prostaglandin E Receptor 2 (PTGER2/EP2 Receptor) Stable Cell Line

Prostaglandin E Receptor 2 (PTGER2), commonly known as the EP2 receptor, is a Class A G protein-coupled receptor (GPCR) that serves as one of the four receptors for prostaglandin E2 (PGE2), a major lipid mediator involved in inflammation, immune regulation, pain signaling, reproduction, and tissue homeostasis. EP2 is widely expressed in immune cells, vascular tissues, reproductive organs, bone, kidney, lung, and the central nervous system. Upon activation by PGE2, PTGER2 primarily couples to Gs proteins, stimulating adenylate cyclase activity and increasing intracellular cAMP levels, thereby regulating numerous downstream signaling pathways associated with cellular growth, differentiation, inflammation, and immune responses.

PTGER2 plays critical roles in modulating inflammatory reactions, vasodilation, bone metabolism, reproductive physiology, and immune cell function. Elevated PGE2-EP2 signaling has been implicated in chronic inflammatory diseases, cancer progression, neurodegenerative disorders, pain, and autoimmune conditions. In oncology, EP2 activation has been associated with tumor growth, angiogenesis, immune suppression, and metastatic progression. Consequently, PTGER2 has emerged as an important therapeutic target for inflammation, cancer, pain management, and immunological disorders.

eEnzyme's Human PTGER2 Stable Cell Line provides a reliable and reproducible cellular platform for investigating EP2 receptor pharmacology and signaling mechanisms. The cell line enables quantitative assessment of agonists, antagonists, inverse agonists, and allosteric modulators through cAMP assays, reporter gene assays, and other GPCR functional screening platforms. Researchers can utilize this stable cell line for receptor characterization, compound profiling, lead optimization, and high-throughput screening applications supporting inflammation research, immunology studies, and drug discovery programs.

Applications of the PTGER2 Stable Cell Line

• EP2 receptor pharmacology studies
• Prostaglandin E2 signaling investigations
• Agonist and antagonist screening
• Inflammation and immunology research
• Cancer biology and tumor microenvironment studies
• Pain and neuroinflammation research
• cAMP and GPCR functional assays
• High-throughput screening applications

Biological Significance of PTGER2

PTGER2 is a major mediator of prostaglandin E2 signaling and contributes to the regulation of numerous physiological and pathological processes. Activation of EP2 stimulates cAMP-dependent signaling pathways that regulate cytokine production, immune cell activation, vascular function, bone remodeling, and tissue repair. In inflammatory diseases, EP2 signaling can influence both innate and adaptive immune responses. In cancer, excessive activation of the PGE2-EP2 axis has been shown to promote tumor growth, angiogenesis, immune evasion, and metastatic potential. EP2 signaling has also been implicated in neurological disorders, pain pathways, and age-related inflammatory conditions. Because of its broad biological functions and therapeutic relevance, PTGER2 remains an important target for drug discovery programs focused on inflammation, oncology, immunology, and neurodegenerative diseases. The PTGER2 Stable Cell Line provides a valuable tool for studying prostaglandin receptor biology and advancing the development of next-generation EP2-targeted therapeutics.