About Human Parathyroid Hormone 2 Receptor (PTH2R) Stable Cell Line

Parathyroid Hormone 2 Receptor (PTH2R) is a Class B G protein-coupled receptor (GPCR) that belongs to the secretin receptor family. Unlike PTH1R, which is activated by both parathyroid hormone (PTH) and parathyroid hormone-related peptide (PTHrP), PTH2R is selectively activated by tuberoinfundibular peptide of 39 residues (TIP39), a neuropeptide that functions as its endogenous ligand. PTH2R is primarily expressed in the central nervous system, endocrine tissues, pancreas, heart, and other peripheral organs. Upon activation, PTH2R primarily couples to Gs proteins, leading to activation of adenylate cyclase, elevation of intracellular cAMP levels, and regulation of downstream signaling pathways involved in neuroendocrine function and cellular communication.

PTH2R plays important roles in pain perception, stress responses, neuroendocrine regulation, reproductive physiology, and emotional behavior. Studies have demonstrated that the TIP39-PTH2R signaling axis contributes to modulation of nociception, anxiety-related behaviors, maternal functions, auditory processing, and hormone secretion. Because of its unique physiological functions and restricted tissue distribution, PTH2R has emerged as an important target for neuroscience research and therapeutic development focused on neurological and neuroendocrine disorders.

eEnzyme's Human PTH2R Stable Cell Line provides a reliable and reproducible cellular platform for investigating PTH2R-mediated signaling pathways and evaluating receptor-targeted therapeutics. The cell line enables quantitative assessment of TIP39 analogs, agonists, antagonists, and novel receptor modulators through cAMP assays, reporter gene assays, and other GPCR functional screening platforms. Researchers can utilize this stable cell line for receptor characterization, ligand profiling, potency determination, lead optimization, and high-throughput screening applications supporting neuroscience and neuroendocrine drug discovery programs.

Applications of the PTH2R Stable Cell Line

• PTH2R receptor pharmacology studies
• TIP39 signaling investigations
• Agonist and antagonist screening
• Neuroendocrine signaling research
• Pain and nociception studies
• Stress and anxiety pathway investigations
• cAMP and GPCR functional assays
• High-throughput screening applications

Biological Significance of PTH2R

PTH2R is a unique member of the parathyroid hormone receptor family with specialized functions in the nervous and endocrine systems. Activation of PTH2R by TIP39 regulates neuronal activity and influences physiological processes including pain modulation, emotional behavior, stress adaptation, reproductive function, and hormone secretion. The receptor is highly expressed in discrete brain regions involved in sensory processing and neuroendocrine regulation, suggesting important roles in central nervous system communication. Dysregulation of the TIP39-PTH2R signaling pathway has been linked to altered pain sensitivity, anxiety-related behaviors, and endocrine dysfunction. Because of its involvement in neurobiology and endocrine regulation, PTH2R remains an important target for neuroscience research and the development of therapeutics targeting neurological, behavioral, and neuroendocrine disorders. The PTH2R Stable Cell Line provides a valuable tool for studying receptor biology and advancing GPCR-focused drug discovery programs.