EK-001P

Name

Recombinant Enterokinase (rEK)

Description

Recombinant Enterokinase (rEK) is the catalytic subunit of bovine enterokinase, which is expressed by the yeast Pichia pastoris and purified to yield a high enzyme activity preparation. rEK recognizes the sequence Asp-Asp-Asp-Asp-Lys and cleaves the peptide bond after the lysine residue. The enzyme can be used to cleave any fusion protein that carries this sequence.

Source

Yeast

Unit Definition

One unit of rEK is the amount of enzyme that will cleave 20µg of thioredoxin-chloramphenicci acetyl transferase fusion protein containing an enterokinase cleavage site (Asp-Asp-Asp-Asp-Lys) to 90% completion at 37℃ in 16 hours

Size

1000 units (1 U/µl)

For Downloading

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