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SARS-CoV-2 Pseudoviral Particles

Catalog Number:
SCV2-PsV-001
SARS-CoV-2 Pseudoviral Particles, replication-deficient MLV pseudotyped with the SARS-CoV-2 spike protein. (Mobile users please click "View Full Site" for details)
Price: $800.00
Detailed Description
Keywords: coronavirus, SARS, SARS-Cov, SARS-COV-2, covid19, covid-19, SARS pseudovirus, sars-cov-2 pseudovirus, sars-cov-2 spike, ace2, SARS-2, new SARS, SARS-Cov-2, nCOV2019, pseudovirus, pseudoviral particle, spike, MLV, LUC

SCV2-PsV-001

Name

SARS-CoV-2 Pseudoviral Particles 

Description

Our SARS-CoV-2 Pseudoviral Particles are replication-deficient Maloneymurine Leukemia Virus (MLV or MuLV) pseudotyped with the SARS-CoV-2 spike protein carrying the original D614 genotype (Genbank Accession # YP_009724390.1). 
MLV-based virus system is a better choice than HIV-based lentivirus system for making pseudoviral particles in regards of lab safety and product quality. Gag-Pol is the only component from MLV.

Applications

Our Pseudovirus Particles generate robust chemiluminescent signals in cell assays when coupled with our firefly luciferase assay kit (Catalog # CA-L165), useful for 1) screening potential inhibitors to block SARS-CoV-2 entry and viral protein; 2) measuring the activity of and screening for neutralizing antibody against SARS-CoV-2. 

Size

10 ml (2 tubes, 5 mL/tube), for 2 multi-well plates. Pseudoviral particles per mL ~ 1.0E+08

Storage

 -70 C. Shipped on dry ice.

Note

1. Amenable to HTS format (96-well, 384-well and 1536-well format). 
2. HEK293-hACE2 stable cell lines are available seperately (Cat..#  CL-hACE2-001, CL-hACE2-002)

For Downloading

==>> DATA SHEET              ==>> SAFETY DATA SHEET

This product has been tested in many COVID research work. Here are a few citations: 

1) A novel linker-immunodominant site (LIS) vaccine targeting the SARS-CoV-2 spike protein protects against severe COVID-19 in Syrian hamsters. Emerging Microbes & Infections, May 2021.
2) Immunogenicity and Neutralizing Activity Comparison of SARS-CoV-2 Spike Full-Length and Subunit Domain Proteins in Young Adult and Old-Aged Mice.  Vaccines, Feb. 2021.
3) Serum Antibody Profile of a Patient With Coronavirus Disease 2019 Reinfection. Clinical Infectious Diseases, Sept. 2020.
4) Metallodrug ranitidine bismuth citrate suppresses SARS-CoV-2 replication and relieves virus-associated pneumonia in Syrian hamsters. Nat. Microbiol. 5, 1439–1448 (2020).

For background reading
:
Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein , Walls, et al, 2020, Cell 181, 281–292

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