SARS-CoV-2 Pseudoviral Particles,Wuhan-Hu-1

Catalog Number:
SCV2-PsV-001
SARS-CoV-2 Pseudoviral Particles, replication-deficient MLV pseudotyped with the SARS-CoV-2 spike protein of the Wuhan-Hu-1 strain. (Mobile users please click "View Full Site" for details)
Price: $400.00
Detailed Description
Keywords: coronavirus, SARS, SARS-Cov, SARS-COV-2, covid19, covid-19, SARS pseudovirus, sars-cov-2 pseudovirus, sars-cov-2 spike, ace2, SARS-2, new SARS, SARS-Cov-2, nCOV2019, pseudovirus, pseudoviral particle, spike, MLV, LUC

SCV2-PsV-001

Name

SARS-CoV-2 Pseudoviral Particles,  Wuhan-Hu-1

Description

Our SARS-CoV-2 Pseudoviral Particles are replication-deficient Maloneymurine Leukemia Virus (MLV or MuLV) pseudotyped with the SARS-CoV-2 spike protein of the original Wuhan strain (the Wuhan-Hu-1 isolate; Genbank Accession # YP_009724390.1). 
MLV-based virus system is a good choice for making pseudoviral particles in regards of lab safety and product quality. 

Applications

Our Pseudovirus Particles generate robust chemiluminescent signals in cell assays when coupled with our firefly luciferase assay kit (Catalog # CA-L165), useful for 1) screening potential inhibitors to block SARS-CoV-2 entry and viral protein; 2) measuring the activity of and screening for neutralizing antibody against SARS-CoV-2. 

Size

5 ml, for one multi-well plate. Pseudoviral particles per mL ~ 1.0E+08

Storage

 -70 C. Shipped on dry ice.

Note

1. Amenable to HTS format (96-well, 384-well and 1536-well format). 
2. HEK293-hACE2 stable cell lines are available seperately (Cat..#  CL-hACE2-001, CL-hACE2-002)

For Downloading

==>> DATA SHEET              ==>> SAFETY DATA SHEET

This product has been used in a lot COVID research work. Here are a few citations: 

1) Development and Characterization of Phage-Display-Derived Novel Human Monoclonal Antibodies against the Receptor Binding Domain of SARS-CoV-2. Biomedicines 2022, 10(12), 3274
2) Identification of SARS-CoV-2 Neutralizing Antibody with Pseudotyped Virus-Based Test on HEK-293T hACE2 Cells. Bio-protocol, April 05, 2022.
3) A novel linker-immunodominant site (LIS) vaccine targeting the SARS-CoV-2 spike protein protects against severe COVID-19 in Syrian hamsters. Emerging Microbes & Infections, May 2021.
4) Immunogenicity and Neutralizing Activity Comparison of SARS-CoV-2 Spike Full-Length and Subunit Domain Proteins in Young Adult and Old-Aged Mice.  Vaccines, Feb. 2021.
5) Serum Antibody Profile of a Patient With Coronavirus Disease 2019 Reinfection. Clinical Infectious Diseases, Sept. 2020.
6) Metallodrug ranitidine bismuth citrate suppresses SARS-CoV-2 replication and relieves virus-associated pneumonia in Syrian hamsters. Nat. Microbiol. 5, 1439–1448 (2020).

For background reading
:
Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein , Walls, et al, 2020, Cell 181, 281–292

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