SARS-CoV-2 Pseudoviral Particles,Wuhan-Hu-1
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Catalog Number:
SCV2-PsV-001
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SARS-CoV-2 Pseudoviral Particles, replication-deficient MLV pseudotyped with the SARS-CoV-2 spike protein of the Wuhan-Hu-1 strain. (Mobile users please click "View Full Site" for details)
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Detailed Description
Keywords: coronavirus, SARS, SARS-Cov, SARS-COV-2, covid19, covid-19, SARS pseudovirus, sars-cov-2 pseudovirus, sars-cov-2 spike, ace2, SARS-2, new SARS, SARS-Cov-2, nCOV2019, pseudovirus, pseudoviral particle, spike, MLV, LUC
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SCV2-PsV-001
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Name
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SARS-CoV-2 Pseudoviral Particles, Wuhan-Hu-1
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Description
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The SARS-CoV-2 Pseudoviral Particles we produce are replication-deficient Maloneymurine Leukemia Virus (MLV or MuLV) pseudotyped with the SARS-CoV-2 spike protein of the original Wuhan strain (the Wuhan-Hu-1 isolate; Genbank Accession # YP_009724390.1).
MLV-based virus system is a good choice for making pseudoviral particles in regards of lab safety and product quality.
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Applications
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Our Pseudovirus Particles generate robust chemiluminescent signals in cell assays when coupled with our firefly luciferase assay kit (Catalog # CA-L165), useful for 1) screening potential inhibitors to block SARS-CoV-2 entry and viral protein; 2) measuring the activity of and screening for neutralizing antibody against SARS-CoV-2.
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Size
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5 ml, for one multi-well plate. Pseudoviral particles per mL ~ 1.0E+08
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Storage
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-70 C. Shipped on dry ice.
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Note
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1. Amenable to HTS format (96-well, 384-well and 1536-well format).
2. HEK293-hACE2 stable cell lines are available seperately (Cat..# CL-hACE2-001, CL-hACE2-002)
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For Downloading
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==>> DATA SHEET ==>> SAFETY DATA SHEET |
This product has been used in a lot COVID research work. Here are a few citations:
1) Development and Characterization of Phage-Display-Derived Novel Human Monoclonal Antibodies against the Receptor Binding Domain of SARS-CoV-2. Biomedicines 2022, 10(12), 3274
2) Identification of SARS-CoV-2 Neutralizing Antibody with Pseudotyped Virus-Based Test on HEK-293T hACE2 Cells. Bio-protocol, April 05, 2022.
3) A novel linker-immunodominant site (LIS) vaccine targeting the SARS-CoV-2 spike protein protects against severe COVID-19 in Syrian hamsters. Emerging Microbes & Infections, May 2021.
4) Immunogenicity and Neutralizing Activity Comparison of SARS-CoV-2 Spike Full-Length and Subunit Domain Proteins in Young Adult and Old-Aged Mice. Vaccines, Feb. 2021.
5) Serum Antibody Profile of a Patient With Coronavirus Disease 2019 Reinfection. Clinical Infectious Diseases, Sept. 2020.
6) Metallodrug ranitidine bismuth citrate suppresses SARS-CoV-2 replication and relieves virus-associated pneumonia in Syrian hamsters. Nat. Microbiol. 5, 1439–1448 (2020).
For background reading: Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein , Walls, et al, 2020, Cell 181, 281–292
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