About Human Vasoactive Intestinal Peptide Receptor 2 (VIPR2/VPAC2) Stable Cell Line

Vasoactive Intestinal Peptide Receptor 2 (VIPR2), also known as VPAC2 receptor, is a Class B G protein-coupled receptor (GPCR) that binds vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating polypeptide (PACAP) with high affinity. VIPR2 is broadly expressed in the central nervous system, endocrine tissues, immune cells, lungs, gastrointestinal tract, pancreas, and cardiovascular system. Upon activation by VIP or PACAP, VIPR2 primarily couples to Gs proteins, stimulating adenylate cyclase activity and increasing intracellular cAMP levels, which regulate numerous downstream signaling pathways involved in neuroendocrine function, immune regulation, metabolism, and circadian rhythm control.

VIPR2 plays essential roles in maintaining physiological homeostasis through its regulation of hormone secretion, immune responses, neuronal signaling, smooth muscle relaxation, and energy metabolism. In the central nervous system, VIPR2 is particularly important for circadian rhythm regulation through signaling within the suprachiasmatic nucleus (SCN), the master biological clock. Dysregulation of VIPR2 signaling has been associated with neuropsychiatric disorders, inflammatory diseases, pulmonary conditions, metabolic dysfunction, and certain cancers. Genetic studies have also linked VIPR2 abnormalities to schizophrenia and other neurological disorders, highlighting its growing importance in neuroscience research and therapeutic development.

eEnzyme's Human VIPR2 Stable Cell Line provides a reliable and reproducible cellular platform for investigating VIPR2-mediated signaling pathways and evaluating receptor-targeted therapeutics. The cell line enables quantitative assessment of VIP analogs, PACAP analogs, agonists, antagonists, antibodies, and novel receptor modulators through cAMP assays, reporter gene assays, and other GPCR functional screening platforms. Researchers can utilize this stable cell line for receptor characterization, ligand profiling, potency determination, lead optimization, and high-throughput screening applications supporting neuroscience, immunology, metabolic disease, and endocrine research.

Applications of the VIPR2 Stable Cell Line

• VIPR2 receptor pharmacology studies
• VIP and PACAP signaling investigations
• Agonist and antagonist screening
• Circadian rhythm and sleep research
• Neuropsychiatric disorder studies
• Immunology and inflammation research
• cAMP and GPCR functional assays
• High-throughput screening applications

Biological Significance of VIPR2

VIPR2 is a critical mediator of VIP and PACAP signaling in both the nervous system and peripheral tissues. Activation of VIPR2 regulates circadian rhythm synchronization, hormone secretion, immune cell activity, smooth muscle relaxation, and metabolic homeostasis. Within the suprachiasmatic nucleus, VIPR2 signaling coordinates neuronal communication required for maintaining normal biological rhythms and sleep-wake cycles. In addition, VIPR2 contributes to anti-inflammatory responses, pulmonary function, gastrointestinal physiology, and endocrine regulation. Emerging evidence has linked altered VIPR2 signaling to schizophrenia, mood disorders, and other neurodevelopmental conditions, making the receptor an increasingly important target for neuroscience research. Because of its broad physiological functions and therapeutic relevance, VIPR2 remains an attractive target for drug discovery programs focused on neurological, inflammatory, metabolic, and endocrine diseases. The VIPR2 Stable Cell Line provides a valuable tool for studying receptor biology and advancing the development of next-generation VIPR2-targeted therapeutics.