Selected Citation:

Wang, J. et al. Anti-Enterovirus 71 Effects of Chrysin and Its Phosphate Ester. PLoS One 9, e89668 (2014).  

About Human Enterovirus A71 (EV71) VP1 Protein

Human Enterovirus A71 (EV71) is a major causative agent of hand, foot and mouth disease (HFMD) and is associated with severe neurological complications including meningitis, encephalitis, and acute flaccid paralysis. The VP1 protein is the largest and most immunogenic structural protein of the EV71 capsid and plays a critical role in viral attachment, receptor recognition, host-cell entry, and viral infectivity. Together with VP2 and VP3, VP1 is exposed on the surface of the viral particle and contributes to the antigenic properties of the virus. Numerous studies have demonstrated that VP1 contains the major neutralizing epitopes recognized by protective antibodies, making it one of the most important targets for vaccine development and immune response studies.

Because of its strong immunogenicity and critical role in viral pathogenesis, VP1 has become one of the most extensively studied EV71 antigens. Multiple vaccine platforms, including recombinant protein vaccines, virus-like particles (VLPs), DNA vaccines, and mRNA vaccines, have incorporated VP1-derived antigens or epitopes to induce protective immune responses. Several neutralizing epitopes have been identified within VP1, including regions involved in antibody recognition and cross-protective immunity against different EV71 strains.

eEnzyme's recombinant Human Enterovirus A71 VP1 Protein provides a valuable research tool for studies of viral pathogenesis, vaccine development, antibody characterization, antigen discovery, and host immune responses. The protein is suitable for antibody generation, epitope mapping, structural biology investigations, ELISA development, Western blot analysis, and immunological assay development. Researchers can utilize this recombinant antigen to investigate EV71-specific immunity, characterize neutralizing antibody responses, and support the development of vaccines, diagnostics, and antiviral therapeutics against EV71 infection.

Applications of EV71 VP1 Protein

• Enterovirus A71 vaccine research and development
• Neutralizing antibody discovery and characterization
• Antibody generation and validation
• Epitope mapping and antigenicity analysis
• Viral pathogenesis and immune response studies
• ELISA, Western blot, and immunological assays

Biological Significance of VP1

VP1 is a major surface-exposed capsid protein that plays a central role in EV71 infection and immune recognition. The protein participates in receptor binding, viral entry, and uncoating processes that are essential for initiation of infection. Multiple neutralizing epitopes have been mapped to VP1, including regions within the GH loop that are recognized by protective antibodies. Because VP1 serves as a dominant target of humoral immune responses and contributes significantly to protective immunity, it remains one of the most important antigens for EV71 vaccine development, diagnostic assay design, and antiviral research.