About Anti-p24 (HIV-1/Clade A) Rabbit Polyclonal Antibody

The HIV-1 p24 capsid protein is one of the major structural proteins of the virus and is generated by proteolytic cleavage of the p55 Gag polyprotein during viral maturation. p24 forms the conical viral core that encapsidates the viral RNA genome and associated replication enzymes, making it essential for viral assembly, maturation, infectivity, and early stages of infection. Because p24 is highly conserved, abundantly expressed during viral replication, and strongly immunogenic, it has become one of the most widely used biomarkers for HIV detection, vaccine development, lentiviral vector characterization, and virology research. HIV-1 Clade A is prevalent in parts of East Africa, Central Asia, and Eastern Europe, making Clade A-specific reagents valuable for regional HIV research and vaccine studies.

eEnzyme's Anti-p24 (HIV-1/Clade A) Rabbit Polyclonal Antibody is generated by in vivo genetic immunization using an in vivo expressed HIV-1 Clade A p24 protein (GenBank accession AF084657) as the immunogen. This immunization strategy produces high-affinity antibodies that recognize native p24 and related Gag proteins while preserving conformational epitopes. The antibody is immunogen affinity-purified, specifically reacts with HIV-1 Clade A p24 and p55 Gag proteins, and is suitable for Western blotting, ELISA, immunofluorescence (IF), immunocytochemistry (ICC), immunoprecipitation (IP), and other immunological assays. It provides a valuable reagent for HIV research, recombinant protein characterization, lentiviral vector production, vaccine development, and diagnostic assay development.

Applications

Biological Significance of HIV-1 p24

The HIV-1 p24 capsid protein is indispensable for viral replication and infectivity. Following protease-mediated cleavage of the p55 Gag precursor, p24 assembles into the mature viral capsid, which protects the viral genome and orchestrates reverse transcription, intracellular trafficking, and nuclear import after infection. Because p24 is highly conserved and produced at high levels during active viral replication, it is widely used as a marker for monitoring virus production, quantifying lentiviral vectors, and detecting early HIV infection. The p24 antigen is also extensively utilized in vaccine evaluation, antiviral drug development, and studies of HIV assembly and maturation. Consequently, Anti-p24 (HIV-1/Clade A) Rabbit Polyclonal Antibody provides a robust research tool for detecting native and recombinant p24, monitoring HIV and lentiviral vector production, characterizing Gag protein expression, and supporting HIV molecular virology, immunology, and therapeutic development.