About Elite™ cADP-Ribose Assay Kit (Fluorescence)

Cyclic ADP-ribose (cADPR) is a potent intracellular calcium (Ca²⁺) second messenger generated from NAD⁺ by ADP-ribosyl cyclases such as CD38. cADPR regulates intracellular Ca²⁺ release primarily through ryanodine receptors, thereby influencing numerous physiological processes including immune cell activation, insulin secretion, muscle contraction, neuronal signaling, and cell proliferation. Dysregulation of cADPR metabolism has been implicated in cancer, inflammation, metabolic disorders, cardiovascular disease, and viral infections, making it an important biomarker for cell signaling and drug discovery.

eEnzyme's Elite™ cADP-Ribose Assay Kit (Fluorescence) provides an ultrasensitive fluorometric assay for quantifying cADPR in cell and tissue samples. The assay converts cADPR to NAD⁺ through an enzyme-coupled reaction, followed by highly sensitive fluorescence detection, enabling reliable measurement of cADPR at low nanomolar concentrations. The convenient mix-and-read format is compatible with both 96- and 384-well microplates, making it suitable for routine analysis and high-throughput screening applications.

Applications

Biological Significance of cADP-Ribose

cADPR is a key intracellular messenger that links NAD⁺ metabolism to Ca²⁺ signaling. By promoting calcium release from intracellular stores through ryanodine receptors, cADPR regulates diverse cellular processes including immune responses, secretion, muscle function, neuronal activity, and cell survival. Because alterations in cADPR signaling are associated with numerous human diseases, accurate quantification of cADPR is essential for investigating cellular signaling pathways, evaluating CD38-targeted therapeutics, and advancing research in immunology, metabolism, and biomedical sciences.