CA-C325
Name
Elite™ cAMP 384-Well ELISA Kit (Fluorescence)
Description
Elite™ cAMP 384-Well ELISA Kit provides a sensitive method for detecting adenylate cyclase activity in biochemical or cell-based assay system. Compared to other cAMP ELISA kits, our kit eliminates the tedious acetylation step. The kit uses Elite™ Red as a fluorimetric substrate to quantify the HRP activity. The high sensitivity (for as low as 0.1 nM) makes it superior to any other known colorimetric-based or fluorescence-based kits in the market.
Application
The assay can be conveniently performed in a 384-well microtiter-plate format and can be easily adapted to automation. The fluorescent product formed is proportional to the activity of the HRP-cAMP conjugate.
Sensitivity
0.1 pmol/ml to 10 pmol/ml; EC50 = 1.6 pmol/ml
Size
1 plate (384-well)
Detection
Fluorescence microplate reader
For Downloading
===> DATA SHEET
Selected Citations:1. GPR101 mediates the pro-resolving actions of RvD5 n-3 DPA in arthritis and infections. J. Clin. Invest. 130, 359–373 (2020). 2. Effect of XingPiJieYu decoction on spatial learning and memory and cAMP-PKA-CREB-BDNF pathway in rat model of depression through chronic unpredictable stress. BMC Complement. Altern. Med. 17, 1–12 (2017).3. Novel resolvin D2 receptor axis in infectious inflammation. J. Immunol. 198, 842–851 (2017).