Cell Cytotoxicity Assay Kit (Red Fluorescence)
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Catalog Number:
CA-C066
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Detailed Description
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CA-C066
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Name
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Elite™ Cell Cytotoxicity Assay Kit (Red Fluorescence)
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Description
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This assay is more sensitive for cell proliferation and cytotoxicity than many other assays. The kit components are quite stable with minimal cytotoxicity, thus a longer incubation time (i.e. 24 to 48 hours) is possible if required.
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Application
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Excellent for high throughput screening of cell proliferation or cytotoxicity against a variety of compounds.
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Size
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100 assays (96-well plate)
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Ex/Em
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540/590 nm
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Detection
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Fluorescence microplate reader
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For Downloading
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==>> DATA SHEET ==>> MSDS
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About Elite™ Cell Cytotoxicity Assay Kit (Red Fluorescence)
Cytotoxicity assays are essential tools for evaluating the effects of drugs, biologics, environmental toxins, and immune cells on cell survival. Loss of plasma membrane integrity is one of the earliest and most reliable indicators of irreversible cell injury and death. Fluorescent cytotoxicity assays utilize membrane-impermeable nucleic acid dyes that selectively enter cells with compromised membranes, where they bind DNA and produce a strong fluorescent signal. Because viable cells exclude these dyes, fluorescence intensity is directly proportional to the number of dead or damaged cells, providing a rapid and quantitative measure of cytotoxicity.
eEnzyme's Elite™ Cell Cytotoxicity Assay Kit (Red Fluorescence) provides a rapid, sensitive, and homogeneous method for quantifying dead cells using a proprietary red fluorescent nucleic acid dye. The dye is excluded by healthy cells but readily penetrates cells with compromised plasma membranes, producing bright red fluorescence upon binding intracellular nucleic acids. The assay requires only a simple mix-and-read procedure with no wash or separation steps and is compatible with both adherent and suspension cells. Optimized for 96- and 384-well microplates, the kit is ideal for fluorescence microplate readers, automated imaging systems, and high-throughput screening (HTS).
Applications
• Cell cytotoxicity determination
• Drug toxicity and safety evaluation
• Anticancer compound screening
• Immune cell-mediated cytotoxicity assays
• High-throughput screening (HTS)
• Cell death quantification
• Combination therapy evaluation
• Cancer, immunology, and toxicology research
Biological Significance of Cell Cytotoxicity Assays
Accurate measurement of cytotoxicity is fundamental for understanding how chemical compounds, therapeutic antibodies, immune effector cells, pathogens, and environmental stressors affect cell survival. Plasma membrane disruption represents a late-stage event in apoptosis and a defining feature of necrotic cell death, making membrane-impermeable fluorescent dyes highly specific indicators of irreversible cellular damage. Fluorescence-based cytotoxicity assays provide excellent sensitivity, a broad dynamic range, and compatibility with automated workflows, enabling rapid evaluation of drug efficacy, therapeutic safety, immune cell killing activity, and cellular responses to experimental treatments. These assays are widely used in cancer biology, immunotherapy development, toxicology, infectious disease research, and pharmaceutical drug discovery.
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