Cell Viability Assay Kit (Green Fluorescence)
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Catalog Number:
CA-C112
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Detailed Description
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CA-C112
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Name
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Elite™ Cell Viability Assay Kit (Green Fluorescence)
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Description
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This kit uses a proprietary green fluorescent dye that easily permeates intact live cells and gets enhanced fluorescence upon entering live cells. The hydrolysis of the non-fluorescent substrate by intracellular esterases generates a strong green fluorescent hydrophilic product that is well-retained in the cell cytoplasm.
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Application
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It is useful for a variety of studies, including cell adhesion, chemotaxis, multidrug resistance, cell viability, apoptosis and cytotoxicity. The kit is suitable for proliferating and non-proliferating cells.
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Size
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5 plates (96-well)
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Ex/Em
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490/520 nm
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Detection
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Fluorescence microplate reader
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For Downloadiing
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==>> DATA SHEET ==>> MSDS
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About Elite™ Cell Viability Assay Kit (Green Fluorescence)
Cell viability is a fundamental indicator of cellular health, membrane integrity, and metabolic activity, making it one of the most widely measured parameters in cell biology, toxicology, and drug discovery. Fluorescent viability assays offer a rapid and sensitive method for distinguishing live cells from damaged or dead cells based on intracellular esterase activity. Cell-permeable fluorogenic substrates readily enter intact cells and are hydrolyzed by intracellular esterases into highly fluorescent products that are retained within the cytoplasm. Because esterase activity is maintained only in metabolically active cells, fluorescence intensity directly correlates with the number of viable cells.
eEnzyme's Elite™ Cell Viability Assay Kit (Green Fluorescence) utilizes a proprietary green fluorescent dye that efficiently permeates live cells and is converted by intracellular esterases into a bright green fluorescent product (Ex/Em = 490/520 nm). The homogeneous assay provides rapid, sensitive detection of viable cells without radioactive reagents or complex procedures. Suitable for both adherent and suspension cells, the kit is compatible with fluorescence microplate readers and fluorescence microscopes, making it ideal for routine laboratory applications as well as high-throughput screening (HTS).
Applications
• Cell viability determination
• Cell proliferation and growth studies
• Cytotoxicity assessment
• Drug discovery and compound screening
• Cell adhesion and migration assays
• Apoptosis research
• Multidrug resistance studies
• High-throughput screening (HTS)
Biological Significance of Cell Viability Assays
Accurate measurement of cell viability is essential for evaluating cellular responses to biological stimuli, genetic manipulation, and therapeutic compounds. Fluorescence-based viability assays provide a direct assessment of metabolically active cells by measuring intracellular esterase activity while preserving cell morphology and minimizing assay complexity. Compared with traditional colorimetric methods, fluorescent viability assays offer higher sensitivity, broader dynamic range, and compatibility with automated imaging and high-throughput workflows. They are widely used to monitor cell health, optimize culture conditions, evaluate cytotoxic effects, and quantify drug efficacy in cancer research, stem cell biology, immunology, regenerative medicine, and pharmaceutical development.
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