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Cell Lines and Pseudoviral Particles

Coronavirus Research Tools > SARS-CoV-2 Pseudoviruses and Related Cell Lines


What are viral pseudotyped particles?
“Viral pseudotyped particles (PP) are enveloped virus particles that harbor heterologous envelope glycoproteins on their surface and a genome lacking essential gene. ...They have proven to be very useful tools used in research with many applications, such as enabling the study of entry pathways of enveloped viruses … ” [Jean Kaoru Millet, etc. Bio Protoc. 2016 Dec 5;6(23): e2035]



                             Figure 1. Outline of the production of the MLV-based spike-pseudotyped particles.

                          (Reference: Construction and applications of SARS-CoV-2 pseudoviruses: a mini reviewInt J Biol Sci. 2021 Apr 10;17(6):1574-1580)


We have generated replication-deficient MLV-based Coronavirus Spike-pseudotyped particles for critically important SARS-CoV-2 spike variants. MLV or MuLV (murine leukemia virus) -based virus system is a better choice than HIV-based lentivirus system for making pseudoviral particles in regards of lab safety and product quality. Gag-Pol is the only component from MLV.

    













Figure 2. . Illustration of the replication-deficient MLV particle pseudotyped with SARS-CoV-2 Spike protein.

           

Figure 3. EM views of our MLV-based spike-pseudotyped particles.  

With our SARS-CoV-2 spike PP items and stable cell lines (i.e. HEK293-ACE2, vero, CHO), researchers could do a lot:

      1) study the interactions between Spike and the host cell receptor(s) in specific conditions;
      2) validate the candidates that blocks the interaction between Spike and the host cell receptor(s);
      3) screen convalescent serum samples for neutralizing antibodies, etc.

Selected citations of our MLV-based SARS-CoV-2 spike-pseudotype particles:
1.  Immunogenicity and Neutralizing Activity Comparison of SARS-CoV-2 Spike Full-Length and Subunit Domain Proteins in Young Adult and Old-Aged Mice. Vaccines (Basel). 2021 Apr; 9(4): 316
2. A novel linker-immunodominant site (LIS) vaccine targeting the SARS-CoV-2 spike protein protects against severe COVID-19 in Syrian hamsters. Emerg Microbes Infect. 2021; 10(1): 874–884
3. Serum Antibody Profile of a Patient With Coronavirus Disease 2019 Reinfection. Clinical Infectious Diseases, 2021, 72(10), e659–e662
4. Metallodrug ranitidine bismuth citrate suppresses SARS-CoV-2 replication and relieves virus-associated pneumonia in Syrian hamsters. Nat. Microbiol. 5, 1439–1448 (2020)

 

Displaying products 1 - 23 of 23 results
MERS-CoV Pseudoviral Particles
MERS-PsV-001
$900.00
MLV-Luciferase Pseudoviral Particles
PsV-001
$200.00
SARS Coronavirus Pseudoviral Particles
SCV1-PsV-003
$900.00
SARS-CoV-2 Pseudoviral Particles
SCV2-PsV-001
$800.00
SARS-CoV-2 Pseudoviral Particles, 614D
SCV2-PsV-0011
$500.00
SARS-CoV-2 Pseudoviral Particles, D614G
SCV2-PsV-614G
$900.00
SARS-CoV-2 Pseudoviral Particles, D614G-A222V
SCV2-PsV-222V
$900.00
SARS-CoV-2 Pseudoviral Particles, D614G-N501Y
SCV2-PsV-501Y
$900.00
SARS-CoV-2 Pseudoviral Particles, D614G-Y453F
SCV2-PsV-453F
$900.00
SARS-CoV-2 Pseudoviral Particles, Omicron BA.2 Variant
SCV2-PsV-OmiBA2
$950.00
SARS-CoV-2 Pseudoviral Particles, Omicron Variant
SCV2-PsV-Omicron
$950.00
Firefly Luciferase Assay Kit
Starting at $79.00
Human Angiotensin II Receptor Type 1 (AGTR-1, AT1R) Stable Cell Line
CL-AT1R-001
Please inquire order@eEnzyme.com
Human Angiotensin-Converting Enzyme 2 (ACE2) Stable Cell Line
CL-hACE2-002
Please inquire order@eEnzyme.com
Human Angiotensin-Converting Enzyme 2 (hACE2) Stable Cell Line
CL-hACE2-001
Please inquire order@eEnzyme.com
Human Neuropilin-1 (NRP-1) Stable Cell Line
CL-NRP1-001
Please inquire order@eEnzyme.com
Human Transmembrane Protease, Serine 2 (TMPRSS-2) Stable Cell Line
CL-TMPRSS2-001
Please inquire order@eEnzyme.com