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Guide for selecting Taq DNA Polymerases for Nucleic Acid Amplification

 

Polymerase Chain Reaction (PCR), invented by Kary B. Mullis, at the Cetus Corporation, who was awarded the 1993 Nobel Prize for chemistry for PCR, is a technique to exponentially amplify in vitro a small quantity of a specific nucleotide sequence using a thermostable (Taq) DNA polymerase. The DNA polymerase reads the template and incorporates a complimentary nucleotide yielding a newly assembled complimentary strand.

 

eENZYME provides high quality and innovative Taq DNA Polymerases for the amplification of any DNA sequences from mammalians, plants, bacteria, or virus. eENZYME Taq DNA Polymerases have been specifically modified or proportionally mixed to fit for various DNA amplifications at variety of conditions.

 

The following tables are used as references for you to select a specific Taq DNA Polymerase/PCR Ready Mix for your various PCR amplifications.

 

Catalogue#

Product

Fidelity

Specificity

GC-rich Amplifying

Hot Start

Amplifying Size (genomic DNA)

Applications

GC-002-0050

BioTherm Taq

++++

++++

No

No

<10 kb

Highest quality standard Taq

DP-008-0250

HotStart PCR Master Mix

+++++

+++++

Yes

Yes

<10 kb

Hotstart applications

DP-005-0050

EU-Taq

++++

++++

Yes

No

<5 kb

RAPD-PCR; High-throughput genptyping/screening; Multiplexed genotyping; Quantitative real-time PCR; Nested PCR; low-error rate, large amplication cycles; highly pure

GC-018-5000

KlenThermase DNA Polymerase

+++++

+++++

Yes

No

<5 kb

Cycle sequencing with dideoxynucleotides; heat stable; short PCR

SD-008-1000

SD Polymerase

++++++

+++++

Yes

No

<30 kb

Isothermal; Strand displacement; LAMP (Loop Mediated Amplification); WGA (Whole Genome Amplification); RCA (Rolling Circle Amplification); MDA (Multiple Displacement Amplification); PCDR; Standard PCR; Multiplex PCR; Long-range PCR up to 30 kb; Real-time PCR (with intercalating dyes only)

SD-028-1000

Hot Start SD Polymerase

++++++

++++++

Yes

Yes

<30 kb

Same as the above for SD Polymerase


Reference: PCR Protocol