MERS CoV Spike (S1) Protein
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Catalog Number:
MERS-S1-005P
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Middle Eastern Respiratory Disease (MERS) full-length spike protein (S1), C-terminal 6xHis-tagged, expressed and purified from 293 cells.
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Detailed Description
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MERS-S1-005P
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Name
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Full-length spike protein (S1) of MERS-CoV
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Description
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C-terminal 6x His tagged S1 protein (amino acid 1-725) of MERS-CoV (GenBank accession# AHY21469.1)
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Source
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Glycosylated recombinant full-length protein expressed and purified from 293 cells
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Application
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Western Blot, antigen, ELISA, etc.
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Purity
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> 95% (by SDS-PAGE)
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Endotoxin
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<0.01 EU per µg of purified protein by LAL test
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Size
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50 µg
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For Downloading
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==>> DATA SHEET ==>> MSDS
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About Middle East Respiratory Syndrome Coronavirus (MERS-CoV) Spike Protein
Middle East Respiratory Syndrome Coronavirus (MERS-CoV) is an enveloped, positive-sense single-stranded RNA virus belonging to the genus Betacoronavirus. The viral Spike (S) glycoprotein is the major surface protein responsible for host-cell attachment, receptor recognition, membrane fusion, and viral entry. The spike protein consists of two functional subunits: S1, which contains the receptor-binding domain (RBD) that binds the cellular receptor DPP4/CD26, and S2, which mediates membrane fusion.
Because the spike protein is the primary target of neutralizing antibodies and protective immune responses, it is widely used in coronavirus research, vaccine development, receptor-binding studies, and antiviral drug discovery. eEnzyme's recombinant MERS-CoV Spike Protein is expressed for research use and is suitable for receptor-binding assays, antibody screening, ELISA development, epitope mapping, structural studies, and vaccine antigen evaluation.
Applications
• Coronavirus vaccine development
• Neutralizing antibody screening
• DPP4/CD26 receptor-binding studies
• Viral entry and membrane fusion research
• ELISA and immunological assay development
• Epitope mapping and structural biology
• Antiviral drug discovery
• Coronavirus-host interaction studies
Biological Significance of the MERS-CoV Spike Protein
The MERS-CoV spike protein mediates viral attachment and entry by binding the DPP4/CD26 receptor on susceptible host cells. As the major surface antigen, it contains the principal neutralizing epitopes recognized by protective antibodies and remains a key target for vaccine development, therapeutic antibody discovery, and antiviral research. Recombinant MERS-CoV Spike Protein provides a valuable reagent for investigating coronavirus biology, receptor interactions, immune responses, and the development of diagnostics and next-generation therapeutics.
Relevant references:
1. Coronavirus Spike Protein and Tropism Changes
2. Structure, Function, and Antigenicity of the SARSCoV-2 Spike Glycoprotein
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