Muscarinic Acetylcholine Receptor M4 (CHRM4) ACTOne Stable Cell Line
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Catalog Number:
CL-01-CHRM4
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In Stock
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Price: Please inquire order@eEnzyme.com
Detailed Description
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CL-01-CHRM4
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Name
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Muscarinic Acetylcholine Receptor M4 (CHRM4) ACTOne™ Stable Cell Line
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Description
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This cell line is a HEK-293-CNG cell line that expresses a recombinant human CHRM4. HEK-293-CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux and cell membrane depolarization.
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Application
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cAMP dependent human CHRM4 cell-based assay;
cell-based high-throughput screening of human CHRM4 agonist/antagonist.
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Size
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1x 106 cells
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Detection
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FlexStation or Microplate reader
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For Downloading
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==>> DATA SHEET ==>> MSDS
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About Human Muscarinic Acetylcholine Receptor M4 (CHRM4/M4R) Stable Cell Line
Muscarinic Acetylcholine Receptor M4 (CHRM4), also known as the M4 muscarinic receptor (M4R), is a Class A G protein-coupled receptor (GPCR) that is activated by the endogenous neurotransmitter acetylcholine. CHRM4 primarily couples to Gi/o proteins, resulting in inhibition of adenylate cyclase activity, reduced intracellular cAMP production, and modulation of ion channel activity. The receptor is highly expressed in the striatum, cerebral cortex, hippocampus, and other regions of the central nervous system, where it regulates cholinergic and dopaminergic neurotransmission, motor function, cognition, and synaptic plasticity.
CHRM4 has emerged as an important therapeutic target for schizophrenia, Parkinson's disease, substance use disorders, Alzheimer's disease, and other neurological disorders. Selective M4 agonists and positive allosteric modulators (PAMs) have attracted significant interest because they may provide antipsychotic efficacy while reducing the adverse effects associated with conventional dopamine receptor antagonists.
eEnzyme's Human CHRM4 Stable Cell Line provides a reliable platform for investigating M4 receptor-mediated signaling and evaluating agonists, antagonists, and positive allosteric modulators (PAMs) using cAMP and other GPCR functional assays. The cell line is well suited for receptor characterization, compound profiling, potency determination, and high-throughput drug screening.
Applications
• CHRM4 (M4R) receptor pharmacology studies
• Cholinergic signaling research
• Agonist, antagonist, and PAM screening
• Schizophrenia drug discovery
• Parkinson's disease research
• CNS and neuropsychiatric disorder studies
• cAMP and GPCR functional assays
• High-throughput screening (HTS)
Biological Significance of CHRM4
CHRM4 is a key regulator of cholinergic signaling in the central nervous system. Activation of the receptor inhibits intracellular cAMP through Gi/o-mediated signaling and modulates the release of acetylcholine and dopamine, particularly within the striatum. Through these actions, CHRM4 contributes to motor control, cognition, learning, memory, and reward-related behaviors. Because of its ability to regulate dopaminergic neurotransmission, CHRM4 has become an attractive target for the development of novel therapies for schizophrenia, Parkinson's disease, addiction, and other CNS disorders. The CHRM4 Stable Cell Line provides a valuable tool for studying muscarinic receptor biology and accelerating the discovery of next-generation therapeutics targeting cholinergic signaling.
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