CA-C166

Name

Elite™ Calcium Dye Non-Wash Ratiometric Assay (Fluorescence)

Description

This kit allows homogeneous measurement of intracellular calcium change caused by activation of G-protein coupled receptors or calcium channel. The ratio of 340/380 nm excitation allows accurate measurements of the intracellular Ca²⁺ concentration. Measuring by ratio considerably reduces the effects of uneven dye loading, leakage of dye, and photo-bleaching, as well as problems associated with measuring Ca²⁺ in cells of unequal thickness.&nThis assay involves only one step of dye addition and does not require any washing steps.

Application

This assay can be conveniently performed in a 96-well or 384-well microtiter-plate format and easily adapted to automation.

Size

Reagents for 10 plates (96-well or 384-well)

Detection

Fluorescence microplate reader

For Downloading

==>> DATA SHEET