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Calcium Dye Non-Wash Ratiometric Assay (Fluorescence)

Catalog Number:
CA-C166
Price: $650.00
Detailed Description

CA-C166

Name

EliteTM Calcium Dye Non-Wash Ratiometric Assay (Fluorescence)

Description

This kit allows homogeneous measurement of intracellular calcium change caused by activation of G-protein coupled receptors or calcium channels. The ratio of 340/380 nm excitation allows accurate measurements of the intracellular Ca2+ concentration. Measuring by ratio considerably reduces the effects of uneven dye loading, leakage of dye, and photo-bleaching, as well as problems associated with measuring Ca2+ in cells of unequal thickness. This assay involves only one step of dye addition and does not require any washing steps.

Application

This assay can be conveniently performed in a 96-well or 384-well microtiter-plate format and easily adapted to automation.

Size

Reagents for 10 plates (96-well or 384-well)

Detection

Fluorescence microplate reader

 

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