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Mitochondrial ROS Activity Assay Kit (Deep Red Fluorescence)

Catalog Number:
CA-R933
Price: $299.00
Detailed Description

CA-R933

Name

EliteTM Mitochondrial ROS Activity Assay Kit  (Deep Red Fluorescence)

Description

This Fluorimetric ROS Assay Kit uses our unique ROS sensor to quantify mitochondrial ROS in live cells. Elite™ ROS deep Red is membrane permeable non-fluorescent ROS sensor. It quickly penetrates into mitochondria and generates very strong fluorescence signal when it reacts with ROS. The kit is an optimized “mix and read” assay format that is compatible with HTS liquid handling instruments.

Application

The kit provides a sensitive, non-wash, one-step fluorimetric assay to detect mitochondrial ROS (especially superoxide and hydroxyl radicals) in live cells within one hour.

Size

200 assays

Detection

Microplate reader (Ex/Em = 650/675 nm); Fluorescence microscope; Flow cytometer

For Downloading

===> DATA SHEET     ===> MSDS



Selected Citations:
1) Adipose stem cell secretome markedly improves rodent heart and human induced pluripotent stem cell-derived cardiomyocyte recovery from cardioplegic transport solution exposure. Stem Cells Journal, Nov. 2020.
2) Effect of cellular and ECM aging on human iPSC-derived cardiomyocyte performance, maturity and senescence. Biomaterials 268, 120554 (2021).
3) Comparative effects of alpha-and gamma-tocopherol on mitochondrial functions in Alzheimer’s Disease in vitro model. Sci. Rep. 10, 1–14 (2020).

4) In vitro aged, hiPSC-origin engineered heart tissue models with age-dependent functional deterioration to study myocardial infarction.

User's Guide for EliteTM ROS Activity Assay Kits



CA-R900

CA-R911

CA-R922

CA-R933

Ex/Em (nm) 

490/525

520/605

540/570

650/675

Fluorescence
Signal

Green

Red

Orange

Deep Red

Target
ROS

Hydrogen peroxide

superoxide

superoxide,
hydroxyl radicals

superoxide,
hydroxyl radicals

Application

total

total

mitochondria

mitochondria

Signal
Strength

++++

+++

++++++

+++++

Detection

1,2

1,2

1,2,3

1,2,3

   *1-fluorescence microplate reader; 2- fluorescence microscope; 3- flow cytometry