Mitochondrial ROS Activity Assay Kit (Deep Red Fluorescence)
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Catalog Number:
CA-R933
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Detailed Description
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CA-R933
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Name
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Elite™ Mitochondrial ROS Activity Assay Kit (Deep Red Fluorescence)
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Description
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This Fluorimetric ROS Assay Kit uses our unique ROS sensor to quantify mitochondrial ROS in live cells. Elite™ ROS deep Red is membrane permeable non-fluorescent ROS sensor. It quickly penetrates into mitochondria and generates very strong fluorescence signal when it reacts with ROS. The kit is an optimized “mix and read” assay format that is compatible with HTS liquid handling instruments.
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Application
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The kit provides a sensitive, non-wash, one-step fluorimetric assay to detect mitochondrial ROS (especially superoxide and hydroxyl radicals) in live cells within one hour.
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Size
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200 assays
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Detection
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Microplate reader (Ex/Em = 650/675 nm); Fluorescence microscope; Flow cytometer
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For Downloading
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===> DATA SHEET ===> MSDS
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Selected Citations:
1) Adipose stem cell secretome markedly improves rodent heart and human induced pluripotent stem cell-derived cardiomyocyte recovery from cardioplegic transport solution exposure. Stem Cells Journal, Nov. 2020.
2) Effect of cellular and ECM aging on human iPSC-derived cardiomyocyte performance, maturity and senescence. Biomaterials 268, 120554 (2021).
3) Comparative effects of alpha-and gamma-tocopherol on mitochondrial functions in Alzheimer’s Disease in vitro model. Sci. Rep. 10, 1–14 (2020).>
4) In vitro aged, hiPSC-origin engineered heart tissue models with age-dependent functional deterioration to study myocardial infarction.
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User's Guide for EliteTM ROS Activity Assay Kits
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CA-R900
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CA-R911
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CA-R922
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CA-R933
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Ex/Em (nm)
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490/525
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520/605
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540/570
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650/675
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Fluorescence
Signal
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Green
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Red
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Orange
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Deep Red
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Target
ROS
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Hydrogen peroxide
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superoxide
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superoxide,
hydroxyl radicals
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superoxide,
hydroxyl radicals
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Application
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total
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total
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mitochondria
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mitochondria
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Signal
Strength
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++++
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+++
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++++++
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+++++
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Detection
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1,2
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1,2
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1,2,3
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1,2,3
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*1-fluorescence microplate reader; 2- fluorescence microscope; 3- flow cytometry
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