About Intracellular ROS Activity Assay Kit (Fluorescence)

Reactive oxygen species (ROS), including superoxide, hydrogen peroxide, and hydroxyl radicals, are continuously generated during normal cellular metabolism and play essential roles in cell signaling, proliferation, differentiation, apoptosis, and immune responses. Excessive ROS production leads to oxidative stress, which contributes to the pathogenesis of numerous diseases, including cancer, cardiovascular disease, neurodegeneration, diabetes, and inflammatory disorders. Consequently, sensitive detection of intracellular ROS has become a fundamental tool in cell biology, toxicology, and drug discovery research.

eEnzyme's Intracellular ROS Activity Assay Kit (Fluorescence) provides a rapid, sensitive, and convenient fluorometric method for detecting total intracellular ROS in live cells. The cell-permeable fluorescent probe is non-fluorescent until oxidized by intracellular ROS, producing a bright fluorescent signal that can be readily measured using a fluorescence microplate reader, fluorescence microscope, or flow cytometer. The simple mix-and-read format is compatible with high-throughput screening and routine cell-based assays.

Applications

Biological Significance of Intracellular ROS

ROS function as important intracellular signaling molecules under physiological conditions but can cause oxidative damage to proteins, lipids, and nucleic acids when produced in excess. Accurate measurement of intracellular ROS is therefore essential for investigating cellular stress responses, evaluating antioxidant compounds, and understanding the molecular mechanisms underlying disease. The Intracellular ROS Activity Assay Kit (Fluorescence) provides a reliable and convenient tool for monitoring oxidative stress in a wide range of biological and biomedical research applications.

Selected Citations:
1) Adipose stem cell secretome markedly improves rodent heart and human induced pluripotent stem cell-derived cardiomyocyte recovery from cardioplegic transport solution exposure. Stem Cells Journal, Nov. 2020.
2) Effect of cellular and ECM aging on human iPSC-derived cardiomyocyte performance, maturity and senescence. Biomaterials 268, 120554 (2021).
3) Comparative effects of alpha-and gamma-tocopherol on mitochondrial functions in Alzheimer’s Disease in vitro model. Sci. Rep. 10, 1–14 (2020).>
4) In vitro aged, hiPSC-origin engineered heart tissue models with age-dependent functional deterioration to study myocardial infarction.

   *1-fluorescence microplate reader; 2- fluorescence microscope; 3- flow cytometry