Mitochondrial ROS Activity Assay Kit (Orange Fluorescence)
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Catalog Number:
CA-R922
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Minimal order 2 kits
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Detailed Description
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CA-R922
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Name
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Elite™ Mitochondrial ROS Activity Assay Kit (Orange Fluorescence)
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Description
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This Fluorimetric ROS Assay Kit uses our unique ROS sensor to quantify mitochondrial ROS in live cells. Elite™ ROS Orange is membrane permeable non-fluorescent ROS sensor. It quickly penetrates into mitochondria and generates very strong fluorescence signal when it reacts with ROS. The kit is an optimized “mix and read” assay format that is compatible with HTS liquid handling instruments.
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Application
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The kit provides a sensitive, non-wash, one-step fluorimetric assay to detect mitochondrial ROS (especially superoxide and hydroxyl radicals) in live cells within one hour.
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Size
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200 assays
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Detection
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Microplate reader (Ex/Em = 540/570 nm); Fluorescence microscope; Flow cytometer
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For Downloading
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==>> DATA SHEET ==>>MSDS
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User's Guide for Elite™ ROS Activity Assay Kits
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CA-R900
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CA-R911
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CA-R922
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CA-R933
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Ex/Em (nm)
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490/525
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520/605
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540/570
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650/675
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Fluorescence
Signal
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Green
(green)
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Red
(red)
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Orange
(orange)
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Deep Red
(deep red)
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Target ROS
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Hydrogen peroxide
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superoxide
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superoxide,
hydroxyl radicals
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superoxide,
hydroxyl radicals
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Application
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total
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total
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mitochondria
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mitochondria
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Signal
Strength
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++++
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+++
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++++++
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+++++
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Detection
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1,2
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1,2
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1,2,3
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1,2,3
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*1-fluorescence microplate reader; 2- fluorescence microscope; 3- flow cytometry
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