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Mitochondrial ROS Activity Assay Kit (Orange Fluorescence)

Catalog Number:
CA-R922
Minimal order 2 kits
Price: $299.00
Detailed Description

CA-R922

Name

EliteTM Mitochondrial ROS Activity Assay Kit (Orange Fluorescence)

Description

This Fluorimetric ROS Assay Kit uses our unique ROS sensor to quantify mitochondrial ROS in live cells. Elite™ ROS Orange is membrane permeable non-fluorescent ROS sensor. It quickly penetrates into mitochondria and generates very strong fluorescence signal when it reacts with ROS. The kit is an optimized “mix and read” assay format that is compatible with HTS liquid handling instruments.

Application

The kit provides a sensitive, non-wash, one-step fluorimetric assay to detect mitochondrial ROS (especially superoxide and hydroxyl radicals) in live cells within one hour.

Size

200 assays

Detection

Microplate reader (Ex/Em = 540/570 nm); Fluorescence microscope; Flow cytometer

 

Click here for DATA SHEET

Click here for MSDS



User's Guide for EliteTM ROS Activity Assay Kits


CA-R900

CA-R911

CA-R922

CA-R933

Ex/Em (nm) 

490/525

520/605

540/570

650/675

Fluorescence
Signal

Green
(green)

Red
(red)

Orange
(orange)

Deep Red
(deep red)

Target ROS

Hydrogen peroxide

superoxide

superoxide,
hydroxyl radicals

superoxide,
hydroxyl radicals

Application

total

total

mitochondria

mitochondria

Signal
Strength

++++

+++

++++++

+++++

Detection

1,2

1,2

1,2,3

1,2,3

   *1-fluorescence microplate reader; 2- fluorescence microscope; 3- flow cytometry