About Human Neuropeptide Y Receptor Y4 (NPY4R/Y4 Receptor) Stable Cell Line

Neuropeptide Y Receptor Y4 (NPY4R), also known as the Y4 receptor, is a Class A G protein-coupled receptor (GPCR) that belongs to the neuropeptide Y receptor family. NPY4R is preferentially activated by pancreatic polypeptide (PP), although it can also respond to other members of the neuropeptide Y family under certain conditions. Upon ligand binding, NPY4R primarily couples to Gi/o proteins, resulting in inhibition of adenylate cyclase activity, reduction of intracellular cAMP levels, and modulation of downstream signaling pathways involved in appetite regulation, energy homeostasis, gastrointestinal function, and neuroendocrine signaling.

NPY4R is expressed in both central and peripheral tissues, including the hypothalamus, brainstem, gastrointestinal tract, pancreas, and other metabolic organs. Through its interactions with pancreatic polypeptide, NPY4R plays an important role in the regulation of food intake, satiety, body weight control, and digestive physiology. Because of its involvement in energy balance and metabolic regulation, NPY4R has emerged as a promising therapeutic target for obesity, metabolic disorders, diabetes, and appetite-related diseases. The receptor has therefore become an important focus of GPCR pharmacology and drug discovery research.

eEnzyme's Human NPY4R Stable Cell Line provides a reliable and reproducible cellular platform for investigating Y4 receptor pharmacology and signaling mechanisms. The cell line enables quantitative evaluation of agonists, antagonists, inverse agonists, and allosteric modulators through cAMP assays, reporter gene assays, and other GPCR functional screening platforms. Researchers can utilize this stable cell line for receptor characterization, compound profiling, lead optimization, and high-throughput screening applications targeting metabolic and gastrointestinal diseases.

Applications of the NPY4R Stable Cell Line

• NPY4R receptor pharmacology studies
• Pancreatic polypeptide signaling investigations
• Agonist and antagonist screening
• Appetite regulation and obesity research
• Metabolic disease drug discovery programs
• cAMP and GPCR signaling assays
• Compound potency and efficacy determination
• High-throughput screening applications

Biological Significance of NPY4R

NPY4R serves as a key regulator of energy balance and neuroendocrine signaling through its interaction with pancreatic polypeptide. Activation of the Y4 receptor suppresses food intake, promotes satiety, and modulates gastrointestinal and pancreatic functions. The receptor contributes to communication between the gastrointestinal tract and central nervous system, helping coordinate physiological responses involved in feeding behavior and metabolism. Alterations in NPY4R signaling have been implicated in obesity, metabolic syndrome, diabetes, and other disorders associated with energy homeostasis. Because of its therapeutic potential in weight management and metabolic disease treatment, NPY4R remains an important target for GPCR research, metabolic biology studies, and the development of next-generation anti-obesity therapeutics.